Anti-Caspase-9 (I20) Antibody (2418)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF,IP Human
SKU: 2418 Categories: , ,
Overview
Product Name Anti-Caspase-9 (I20) Antibody (2418)
Description Anti-Caspase-9 (I120) Rabbit Polyclonal Antibody
Target Caspase-9 (I20)
Species Reactivity Human
Applications ELISA,WB,ICC,IF,IP
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 299- 318 of human caspase-9 (accession no. P55211).
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes full-length human caspase-9 (46kDa).
Target Name Caspase-9
Target ID Caspase-9 (I20)
Uniprot ID P55211
Alternative Names CASP-9, EC 3.4.22.62, Apoptotic protease Mch-6, Apoptotic protease-activating factor 3, APAF-3, ICE-like apoptotic protease 6, ICE-LAP6 [Cleaved into: Caspase-9 subunit p35; Caspase-9 subunit p10]
Gene Name CASP9
Gene ID 842
Accession Number NP_001220
Biological Function Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Promotes DNA damage-induced apoptosis in a ABL1/c-Abl-dependent manner. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).; Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
Research Areas Apoptosis
Background Cell death signals are transduced by death domain-containing adapter molecules and members of the caspase family of proteases. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3, a protease responsible for proteolytic cleavage of many key proteins involved in apoptosis. Caspase-9 plays a central role in cell death induced by a wide variety of apoptosis induced by a wide variety of apoptosis inducers including TNF , TRAIL, anti-CD95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.
Application Images
Description Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines
Loading: 15 ug of lysates per lane. Antibodies: Caspase 9, 2417 (1 ug/mL), Caspase 9, 2418 (1 ug/mL) and beta-actin (1.5 ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Description Western Blot Validation in Human Cell Lines
Loading: 15 ug of lysates per lane. Antibodies: : Caspase 9, 2418 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Description Western Blot Validation in Mouse Cell Line
Loading: 15 ug of 3T3/NIH cell lysate. Antibodies: : Caspase 9, 2418 (1 ug/mL)), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Description Immunofluorescence Validation of Caspase 9 in K562 Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed K562 cells labeling Caspase 9 with 2418 at 20 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green).
Description Immunofluorescence Validation of Caspase 9 in HeLa Cells
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Caspase 9 with 2418 at 5 ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Description Immunocytochemistry Validation of Caspase 9 in HeLa Cells
Immunocytochemical analysis of HeLa cells using anti-Caspase 9 antibody (2418) at 5 ug/ml. Cells was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunocytochemistry: use at 2ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Whole cell lysate from HeLa cells.
References & Data Sheet