Anti-CaMKII, phosphorylated, Antibody (56454)

$457.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP,ELISA Mouse, Rat
SKU: 56454 Categories: , ,
Overview
Product Name Anti-CaMKII, phosphorylated, Antibody (56454)
Description Anti-CamKII, phosphorylated Mouse Monoclonal Antibody
Target CaMKII, phosphorylated,
Species Reactivity Mouse, Rat
Applications WB,IHC,ICC/IF,IP,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 22B1
Isotype IgG1
Immunogen Synthetic peptide based on sequence from rat/mouse CaMKII alpha (aa 281-294), thiophosphorylated at residue Thr286 conjugated to a protein carrier.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes the phosphorylated form of mouse and rat CaMKII. It does not react with non- phosphorylated CaMKII.
Target Name Calcium/calmodulin-dependent protein kinase type II subunitα
Target ID CaMKII, phosphorylated,
Uniprot ID P11275
Alternative Names CaM kinase II subunitα, CaMK-II subunitα, EC 2.7.11.17
Gene Name Camk2a
Sequence Location Cell junction, synapse, Cell junction, synapse, postsynaptic density, Cell projection, dendritic spine, Cell projection, dendrite
Biological Function Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (PubMed:15312654). Regulates dendritic spine development. Also regulates the migration of developing neurons. Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (By similarity). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (By similarity). {UniProtKB:P11798, UniProtKB:Q9UQM7, PubMed:15312654}.
Research Areas Neuroscience
Background CaMKII, a member of the calcium / calmodulin-activated protein kinas family, functions in neural synapatic stimulation and T-cell receptor signaling. CaMKII is expressed in many tissues, but it is specifically found in the neurons of the forebrain, and its mRNA is found within the dendrites and the soma of the neuron. CaMKII in neurons consists of two subunits of 52 (alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains as well as an ATP-binding domain and a consensus phosphorylation site. Binding of calcium / calmodulin to its regulatory domain releases its auto- inhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286. Autophosphorylation confers enhanced affinity of CaMKII for NMDA receptors in postsynaptic densities.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (56454). Tissue: dissociated hippocampal neurons. Species: Rat. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56454) at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.
Description Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (56454). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56454) at 1:5000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
Description Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (56454). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56454) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Muscle, hair follicle, epidermis. Backskin obtained from transgenic mice.
Description Western Blot analysis of Mouse Ventricle lysates showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 22B1 (56454). Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56454) at 1:1000. Analysis of CaMKII and NFAT phosphorylation in ventricles of 14 day old mice over-expressing CaMK.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~50 kDa is detected.

Immunofluorescence: use at 10ug/mL.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Rat brain tissue extract.
References & Data Sheet