Anti-CaMKII Antibody (56453)

$457.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP,ELISA,RIA Mouse, Rat, Bovine
SKU: 56453 Categories: , ,
Overview
Product Name Anti-CaMKII Antibody (56453)
Description Anti-CaMKII Mouse Monoclonal Antibody
Target CaMKII
Species Reactivity Mouse, Rat, Bovine
Applications WB,IHC,ICC/IF,IP,ELISA,RIA
Host Mouse
Clonality Monoclonal
Clone ID 6G9
Isotype IgG1
Immunogen Partially purified rat CaMKII
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes the phosphorylated and non-phosphorylated forms of mouse, rat, and bovine CaMKII.
Target Name Calcium/calmodulin-dependent protein kinase type II subunitα
Target ID CaMKII
Uniprot ID P11798
Alternative Names CaM kinase II subunitα, CaMK-II subunitα, EC 2.7.11.17
Gene Name Camk2a
Sequence Location [Isoform α KAP]: Cytoplasm, Cell junction, synapse, postsynaptic density, Cell projection, dendritic spine, Cell projection, dendrite
Biological Function Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in synaptic plasticity, neurotransmitter release and long-term potentiation. Member of the NMDAR signaling complex in excitatory synapses, it regulates NMDAR-dependent potentiation of the AMPAR and therefore excitatory synaptic transmission (By similarity). Regulates dendritic spine development. Also regulates the migration of developing neurons (By similarity). Phosphorylates the transcription factor FOXO3 to activate its transcriptional activity (PubMed:23805378). Acts as a negative regulator of 2-arachidonoylglycerol (2-AG)-mediated synaptic signaling via modulation of DAGLA activity (PubMed:23502535). {UniProtKB:P11275, UniProtKB:Q9UQM7, PubMed:23502535, PubMed:23805378}.; [Isoform Alpha KAP]: Has no kinase activity. {PubMed:8524307}.
Research Areas Neuroscience
Background CaMKII, a member of the calcium / calmodulin-activated protein kinas family, functions in neural synapatic stimulation and T-cell receptor signaling. CaMKII is expressed in many tissues, but it is specifically found in the neurons of the forebrain, and its mRNA is found within the dendrites and the soma of the neuron. CaMKII in neurons consists of two subunits of 52 (alpha genes) and 60 kDa (beta genes). CaMKII has catalytic and regulatory domains as well as an ATP-binding domain and a consensus phosphorylation site. Binding of calcium / calmodulin to its regulatory domain releases its auto- inhibitory effect and activates the kinase. This kinase activation results in autophosphorylation at threonine 286. Autophosphorylation confers enhanced affinity of CaMKII for NMDA receptors in postsynaptic densities.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (56453). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol for 10 minutes at -20°C. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56453) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Nuclear Staining.
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (56453). Tissue: dissociated hippocampal neurons. Species: Mouse. Fixation: Cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M sodium phosphate buffer. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56453) at 1:1000 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Mouse IgG (green) at 1:50 for 30 minutes at RT. Magnification: 10X. Courtesy of: Mary Kennedy, Caltech.
Description Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (56453). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56453) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Magnification: 40x.
Description Immunohistochemistry analysis using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (56453). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56453) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Hair follicles, epidermis.
Description Western Blot analysis of Rat brain membrane lysate showing detection of CaMKII protein using Mouse Anti-CaMKII Monoclonal Antibody, Clone 6G9 (56453). Primary Antibody: Mouse Anti-CaMKII Monoclonal Antibody (56453) at 1:1000.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.1-1ug/mL. A band of ~50-60 kDa is detected.

Immunohistochemistry: use at 10ug/mL.RIA: use at 3-5ug/mL.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Rat brain tissue extract.
References & Data Sheet