Anti-CA-125/MUC16 Antibody (3345)

$295.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA,IHC Human
SKU: 3345 Categories: , ,
Overview
Product Name Anti-CA-125/MUC16 Antibody (3345)
Description Anti-CA-125 Mouse Monoclonal Antibody
Target CA-125/MUC16
Species Reactivity Human
Applications WB,ELISA,IHC
Host Mouse
Clonality Monoclonal
Clone ID 325
Isotype IgG1
Immunogen CA-125 purified from ovarian carcinoma cells.
Properties
Form Liquid
Concentration Lot Specific
Formulation 10mM Tris buffer, pH 7.5, 0.15M NaCL, 0.05% sodium azide
Buffer Formulation Tris Buffer
Buffer pH pH 7.5
Buffer Anti-Microbial 0.05% Sodium Azide
Format Purified
Purification Purified by ion exchange chromatography
Specificity Information
Specificity #3330 and #3345 epitope group B (ISOBM* classification). #3346 epitope group A (ISOBM* classification)*International Society of Oncology and Biomarkers
Target Name Mucin-16
Target ID CA-125/MUC16
Uniprot ID Q8WXI7
Alternative Names MUC-16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125
Gene Name MUC16
Gene ID 94025
Accession Number NP_078966
Sequence Location Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. Note=May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
Biological Function Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. {ECO:0000250}.
Research Areas Cancer Research
Background CA-125 (cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125) also known as mucin 16 or MUC16 is encoded by the MUC16 gene in humans. MUC16 is a member of the mucin family of glycoproteins. CA-125 is most consistently elevated in epithelial ovarian cancer, but can be expressed in a number of gynecologic (endometrial, fallopian tube) and non- gynecologic (pancreatic, breast, colon and lung) cancers. It may also be elevated in cases of benign abnormalities such as endometriosis.
Application Images
Description Immunohistochemistry on paraffin-embedded samples, left to right, lung adenocarcinoma, serosal ovarian adenocarcinoma, bladder adenocarcinoma.
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions These antibodies may be used in ELISA and Western blotting to detect CA-125.

Positive control: OVCAR3 ovarian adenocarcinoma cells.Suggested pairs for sandwich ELISA:
References & Data Sheet
References Chen, Y et al. 1999. PNAS (USA) 96: 4443-4448
Chang, KH et al. 1997. PNAS (USA) 94: 9040-9045.