Anti-c-myc Antibody, HRP conjugate (18824P)

$522.00

Host Quantity Applications Species Reactivity Data Sheet  
Goat 100ug WB,IHC,ICC,ELISA C-Myc
SKU: 18824P Categories: , ,
Overview
Product Name Anti-c-myc Antibody, HRP conjugate (18824P)
Description Anti-c-myc HRP Goat Polyclonal Antibody
Target c-myc
Species Reactivity C-Myc
Applications WB,IHC,ICC,ELISA
Host Goat
Clonality Polyclonal
Isotype Whole IgG
Conjugate HRP
Conjugate Ratio HRP, Molar enzyme/antibody ratio = 4:1.
Immunogen Synthetic peptide corresponding to aa 410-419 (EQKLISEEDL) of human myc conjugated to KLH.
Source Escherichia coli
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Buffer Formulation Phosphate Buffered Saline
Buffer Anti-Microbial 0.1% Pro-Clean 400
Buffer Protein Stabilizer 0.2% Bovine Serum Albumin
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Specificity Information
Specificity Antigen: Synthetic peptide corresponding to aa 410-419 (EQKLISEEDL) of human myc conjugated to KLH.
Target Name Myc proto-oncogene protein
Target ID c-myc
Uniprot ID P01106
Alternative Names Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64
Gene Name MYC
Sequence Location Nucleus, nucleoplasm, Nucleus, nucleolus
Biological Function Transcription factor that binds DNA in a non-specific manner, yet also specifically rUniProtKB:P01108, PubMed:20010808, PubMed:24940000, PubMed:25956029}.
Research Areas Epitope Tags
Application Images
Description Detection of c-myc-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, goat anti-Myc Tag antibody 18824P used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 10 seconds.
Additional Information
Handling
Storage This product is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Western Blot: dilute 1:1,000 - 1:30,000

Immunocytochemistry: dilute 1:200 - 1:500ELISA: dilute 1:10,000 - 1:100,000These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet
References Smirnova, O. A., Ivanova, O. N., et al. Oxid Med Cell Longev
. 2016
2016:8341937.