Anti-c-myc Antibody (18824-01)

$418.00

Host Quantity Applications Species Reactivity Data Sheet  
Goat 100ug WB,IP,ICC,ELISA C-Myc
SKU: 18824-01 Categories: , ,
Overview
Product Name Anti-c-myc Antibody (18824-01)
Description Anti-c-myc Goat Polyclonal Antibody
Target c-myc
Species Reactivity C-Myc
Applications WB,IP,ICC,ELISA
Host Goat
Clonality Polyclonal
Immunogen Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.2, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target Name Myc proto-oncogene protein
Target ID c-myc
Uniprot ID P01106
Alternative Names Class E basic helix-loop-helix protein 39, bHLHe39, Proto-oncogene c-Myc, Transcription factor p64
Gene Name MYC
Sequence Location Nucleus, nucleoplasm, Nucleus, nucleolus
Biological Function Transcription factor that binds DNA in a non-specific manner, yet also specifically rUniProtKB:P01108, PubMed:20010808, PubMed:24940000, PubMed:25956029}.
Research Areas Epitope Tags
Background c-myc is an epitope tag often constructed on the N- or C-terminus of a protein to allow for detection and analysis of the protein. The peptide sequence of the myc tag is EQKLISEEDL (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu). The c-myc tag is approximately 1202 Daltons in atomic mass and includes 10 amino acids.
Application Images
Description Detection of c-myc-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-Myc Tag antibody 18824 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000-1:20,000.Detection of c-myc-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18824 diluted 1:25,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500.

Immunoprecipitation: use at 1-4ug/mg lysate

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet