Anti-Bnip3L (IN) Antibody (2289)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human, Mouse
SKU: 2289 Categories: , ,
Overview
Product Name Anti-Bnip3L (IN) Antibody (2289)
Description Anti-Bnip3L (IN) Rabbit Polyclonal Antibody
Target Bnip3L (IN)
Species Reactivity Human, Mouse
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 77- 92 of human Bnip3L (accession no. AF067396) This amino acid sequence is identical to that of mouse.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes monomeric human and mouse Bnip3L. A band of 40kDa is detected in immunoblots.
Target Name BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
Target ID Bnip3L (IN)
Uniprot ID O60238
Alternative Names Adenovirus E1B19K-binding protein B5, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A, NIP3-like protein X, NIP3L
Gene Name BNIP3L
Gene ID 665
Accession Number NP_004322
Sequence Location Nucleus envelope. Endoplasmic reticulum. Mitochondrion outer membrane. Membrane
Biological Function Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor. {PubMed:10381623, PubMed:21264228}.
Research Areas Apoptosis
Background A novel BH3 domain-containing protein was recently identified and designated Bnip3L, Bnip3 , or Nix. Bnip3L is a homolog of the E1B 19K/Bcl-2 binding and pro-apoptotic protein Bnip3. Overexpression of Bnip3L induces apoptosis. Bnip3L interacts with and overcomes suppression by Bcl-2 and Bcl-xL. Bnip3L is localized in mitochondria. The messenger RNA of Bnip3L is ubiquitously expressed in human tissues. Bnip3L and Bnip3 form a new subfamily of the pro-apoptotic-mitochondrial proteins.
Application Images
Description Western blot analysis of Bnip3L in K562 whole cell lysate in (A) the absence, or (B) presence of immunogenic peptide with Bnip3L antibody at 1 ug/mL.
Description Immunohistochemical staining of human kidney tissue using Bnip3L antibody at 2 ug/mL.
Description Immunofluorescence of Bnip3L in Human Kidney tissue with Bnip3L antibody at 10 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunohistochemistry: use at 2ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Whole cell lysate of human K562 cells.
References & Data Sheet