Anti-BACE2 (NT) Antibody (2247)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P Human, Mouse, Rat
SKU: 2247 Categories: , ,
Overview
Product Name Anti-BACE2 (NT) Antibody (2247)
Description Anti-BACE2 (NT) Rabbit Polyclonal Antibody
Target BACE2 (NT)
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 44-59 of human BACE2.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat BACE2 (approx. 75 kD).
Target Name β-secretase 2
Target ID BACE2 (NT)
Uniprot ID Q9Y5Z0
Alternative Names EC 3.4.23.45, Aspartic-like protease 56 kDa, Aspartyl protease 1, ASP1, Asp 1, β-site amyloid precursor protein cleaving enzyme 2, β-site APP cleaving enzyme 2, Down region aspartic protease, DRAP, Memapsin-1, Membrane-associated aspartic protease 1,θ-secretase
Gene Name BACE2
Gene ID 25825
Accession Number NP_036237
Sequence Location Cell membrane, Golgi apparatus, Endoplasmic reticulum. Endosome.
Biological Function Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves APP, between residues 690 and 691, leading to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. It has also been shown that it can cleave APP between residues 671 and 672. Responsible also for the proteolytic processing of CLTRN in pancreatic beta cells (PubMed:21907142). {PubMed:10591213, PubMed:11083922, PubMed:11423558, PubMed:15857888, PubMed:16816112, PubMed:21907142}.
Research Areas Neuroscience
Background Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Abeta peptide is generated as a result of proteolytic cleavage of the beta-amyloid protein precursor (APP) at beta- and g-sites. beta-secretase was recently identified and designated beta-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2). A BACE homolog was recently identified and designated BACE2, or Asp1, DRAP (for Down region aspartic protease), or memapsin 1. BACE2 cleaves APP at the beta- site and at a different site within Abeta. BACE2 is found on chromosome 21q22 3, the ‘Down critical region', suggesting that BACE2 and Abeta may also contribute to the pathogenesis of Down syndrome.
Application Images
Description Western blot analysis of BACE2 in (A) human and (B) mouse heart tissue lysates with BACE2 antibody at 1 ug/mL.
Description Immunohistochemistry of BACE2 in mouse heart with BACE2 antibody at 10 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution.

Positive control: Human heart tissue lysate.
References & Data Sheet