Anti-AU1 Antibody (18818-01)

$418.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IP,ICC,ELISA Au1
SKU: 18818-01 Categories: , ,
Overview
Product Name Anti-AU1 Antibody (18818-01)
Description Anti-AU1 Rabbit Polyclonal Antibody
Target AU1
Species Reactivity Au1
Applications WB,IP,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Immunogen AU1 (DTYRYI) conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (PBS), pH 7.2, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity Antibody was affinity-purified on the peptide.
Target ID AU1
Research Areas Epitope Tags
Background AU1 is an epitope tag often constructed on the N- or C- terminus of a protein to allow for detection and analysis of the protein. The AU1 sequence represents the amino acid sequence DTYRYI derived from the major capsid protein of bovine papillomavirus-1 (BPV-1).
Application Images
Description Detection of AU1-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, rabbit anti-AU1 antibody 18818 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000-1:20,000.

Immunocytochemistry: use at a dilution of 1:100-1:400.

ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500.

Immunoprecipitation: use at 1-4ug/mg lysate

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet