Anti-ATM Antibody (13012)

$518.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Goat	100ug	WB,IP	Human, Mouse

SKU: 13012 Categories: Antibody Products, Enzymes and Enzyme Inhibitor Antibodies, Products

Overview

Product Name Anti-ATM Antibody (13012)

Description Anti-ATM Goat Polyclonal Antibody

Target ATM

Species Reactivity Human, Mouse

Applications WB,IP

Host Goat

Clonality Polyclonal

Isotype Whole IgG

Immunogen Synthetic peptide representing a portion of the protein encoded in part by exons 37 and 38 (LocusLink ID 472).

Properties

Form Liquid

Concentration Lot Specific

Formulation Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide

Buffer Formulation Tris

Buffer pH pH 7.8

Buffer Anti-Microbial 0.1% Sodium Azide

Format Purified

Purification Purified by immunoaffinity chromatography

Specificity This antibody reacts with human and mouse. Antibody is affinity purified.

Target Name Serine-protein kinase ATM

Target ID ATM

Uniprot ID Q13315

Alternative Names EC 2.7.11.1, Ataxia telangiectasia mutated, A-T mutated

Gene Name ATM

Accession Number NP_000042

Sequence Location Nucleus, Cytoplasmic vesicle, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome

Biological Function Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. RPubMed:10550055, PubMed:10766245, PubMed:10802669, PubMed:10839545, PubMed:10910365, PubMed:10973490, PubMed:11375976, PubMed:12086603, PubMed:12556884, PubMed:14871926, PubMed:15448695, PubMed:15456891, PubMed:15916964, PubMed:16086026, PubMed:16858402, PubMed:17923702, PubMed:19431188, PubMed:19965871, PubMed:26774286, PubMed:29203878, PubMed:30612738, PubMed:30886146, PubMed:9733514, PubMed:9733515, PubMed:9843217}.

Research Areas Enzymes

Description Detection of human ATM by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified goat anti-ATM antibody 13012 (lot 13012-2) used for IP at 3 µg per reaction. ATM was also immunoprecipitated by goat anti-ATM antibody 13014. For blotting immunoprecipitated ATM, 13012 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Description Detection of human ATM by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-ATM antibody 13012 (lot 13012-2) used for WB at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Handling

Storage Store at 2 - 8°C. Antibody is stable at 2 - 8°C for 1 year.

Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.

Application Instructions Western Blot: 0.2 - 1 ug/ml.

Immunoprecipitation: 10 - 20 ug/mg lysate

Data Sheet