Anti-ATF2 (Phospho-Thr71 or 53) Antibody (43031)
$366.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Rabbit | 100ul | WB,IHC | Human, Mouse, Rat |  |
Overview
Product Name Anti-ATF2 (Phospho-Thr71 or 53) Antibody (43031)
Description Anti-ATF2 (Phospho-Thr71 or 53) Rabbit Polyclonal Antibody
Target ATF2 (Phospho-Thr71 or 53)
Species Reactivity Human, Mouse, Rat
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation sites of threonine 71 or 53 (T-P-T(p)- P-T) derived from human ATF2 and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human, mouse, and rat ATF2 only when phosphorylated at threonine 71 or 53.
Target Name Cyclic AMP-dependent transcription factor ATF-2
Target ID ATF2 (Phospho-Thr71 or 53)
Uniprot ID P15336
Gene Name ATF2
Gene ID 123811
Post Translational Modification ATF2 (Phospho-Thr71 or 53)
Target Phospho-thr71/53
Sequence Location Nucleus. Cytoplasm. Mitochondrion outer membrane. Note=Shuttles between the cytoplasm and the nucleus and heterodimerization with JUN is essential for the nuclear localization. Localization to the cytoplasm is observed under conditions of cellular stress and in disease states. Localizes at the mitochondrial outer membrane in response to genotoxic stress. Phosphorylation at Thr-52 is required for its nuclear localization and negatively regulates its mitochondrial localization. Co-localizes with the MRN complex in the IR-induced foci (IRIF).
Biological Function Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type. {PubMed:10821277, PubMed:15916964, PubMed:18397884, PubMed:22304920}.
Research Areas Phosphospecific Antibodies
Background ATF2 is a transcription factor that is a member of the leucine zipper family of DNA binding protein that can perform multiple functions. It can bind to the cAMP-responsive element (CRE), and it can form a homodimer or a heterodimer with c-Jun and stimulate CRE-dependent transcription. ATF2 is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro. ATF2 may also be involved in a cell's DNA damage response independent of its role in transcriptional regulation.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~65-75kDa is detected.
Immunohistochemistry: use at dilution of 1:50- 1:100.
These are recommended working dilutions.
End user should determine optimal dilutions for their applications.
Immunohistochemistry: use at dilution of 1:50- 1:100.
These are recommended working dilutions.
End user should determine optimal dilutions for their applications.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet