Anti-ARTS Antibody (11000)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human
SKU: 11000 Categories: , ,
Overview
Product Name Anti-ARTS Antibody (11000)
Description Anti-ARTS Rabbit Polyclonal Antibody
Target ARTS
Species Reactivity Human
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 3-14 of human ARTS.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human ARTS (Apoptosis Related Protein in image Signaling Pathway), a novel mito-chondrial septin-like protein. ARTS translocates to the cell nucleus when apoptosis occurs. It enhances apoptosis induced by TGF- and other inducers of apoptosis, such as image and Fas ligand.
Target Name Septin-4
Target ID ARTS
Uniprot ID O43236
Alternative Names Bradeion β, Brain protein H5, CE5B3 β, Cell division control-related protein 2, hCDCREL-2, Peanut-like protein 2
Gene Name SEPTIN4
Gene ID 5414
Accession Number NP_001185642.1
Sequence Location Cytoplasm, Cell projection, cilium, flagellum, Cytoplasmic vesicle, secretory vesicle, Cell projection, axon, Cell projection, dendrite, Perikaryon, Cell junction, synapse
Biological Function Filament-forming cytoskeletal GTPase (Probable). Pro-apoptotic protein involved in LGR5-positive intestinal stem cell and Paneth cell expansion in the intestines, via its interaction with XIAP (By similarity). May also play a role in the regulation of cell fate in the intestine (By similarity). Positive regulator of apoptosis involved in hematopoietic stem cell homeostasis; via its interaction with XIAP (By similarity). Negative regulator of repair and hair follicle regeneration in response to injury, due to inhibition of hair follicle stem cell proliferation, potentially via its interaction with XIAP (By similarity). Plays an important role in male fertility and sperm motility (By similarity). During spermiogenesis, essential for the establishment of the annulus (a fibrous ring structure connecting the midpiece and the principal piece of the sperm flagellum) which is a requisite for the structural and mechanical integrity of the sperm (By similarity). Involved in the migration of cortical neurons and the formation of neuron leading processes during embryonic development (By similarity). Required for dopaminergic metabolism in presynaptic autoreceptors; potentially via activity as a presynaptic scaffold protein (By similarity). {UniProtKB:P28661, PubMed:11146656, PubMed:15029247, PubMed:15837787, PubMed:29020630}.
Research Areas Apoptosis
Application Images
Description Western blot analysis of ARTS expression in human lung (lane 1), spleen (lane 2), and kidney (lane 3) tissue lysates with ARTS antibody at 2 ug/ml.
Description Immunohistochemistry of ARTS in human lung tissue with ARTS antibody at 2 ug/mL.
Description Immunofluorescence of ARTS in Human Lung cells with ARTS antibody at 20 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 2ug/mL. A band of 32 kDa is detected.

Immunohistochemistry: use at 2ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.

Positive control: Human lung, kidney, or spleen lysates.
References & Data Sheet