Anti-ARC (CT) Antibody (2413)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human
SKU: 2413 Categories: , ,
Overview
Product Name Anti-ARC (CT) Antibody (2413)
Description Anti-ARC (CT) Rabbit Polyclonal Antibody
Target ARC (CT)
Species Reactivity Human
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 191- 208 of human ARC.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes full-length ARC (25 kD).
Target Name Nucleolar protein 3
Target ID ARC (CT)
Uniprot ID O60936
Alternative Names Apoptosis repressor with CARD, Muscle-enriched cytoplasmic protein, Myp, Nucleolar protein of 30 kDa, Nop30
Gene Name NOL3
Gene ID 8996
Accession Number NP_003937
Sequence Location [Isoform 1]: Nucleus, nucleolus
Biological Function [Isoform 1]: May be involved in RNA splicing. {PubMed:10196175}.; [Isoform 2]: Functions as an apoptosis repressor that blocks multiple modes of cell death. Inhibits extrinsic apoptotic pathways through two different ways. Firstly by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly (By similarity). SUniProtKB:Q62881, UniProtKB:Q9D1X0, PubMed:15004034, PubMed:15509781}.
Research Areas Apoptosis
Background A novel CARD domain-containing protein was recently identified and designated ARC (apoptosis repressor with CARD). ARC interacts with caspase-2 and -8 and inhibits enzymatic activity of caspase-8. ARC suppresses apoptosis induced by cell death adapters FADD and TRADD and by cell death receptors Fas, TNFR-1, and DR3. The messenger RNA of ARC is primarily expressed in skeletal muscle and cardiac tissue.
Application Images
Description Western blot analysis of ARC in (H) HeLa, (K) K562, and (A) A549 whole cell lysates with ARC antibody at 1 ug/mL.
Description Immunohistochemistry of ARC in human heart tissue with ARC antibody at 5 ug/mL.
Description Immunohistochemistry of ARC in human skeletal muscle with ARC antibody at 5 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/mL.

Immunohistochemistry: use at 5ug/mL.Immunohistochemistry of ARC in human heart tissue

Positive control: Whole cell lysate of HeLa, KB, and A549 cells.
References & Data Sheet