Anti-Apaf1 (NT) Antibody (2412)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,IHC-P,IF Human, Mouse
SKU: 2412 Categories: , ,
Overview
Product Name Anti-Apaf1 (NT) Antibody (2412)
Description Anti-Apaf1 (NT) Rabbit Polyclonal Antibody
Target Apaf1 (NT)
Species Reactivity Human, Mouse
Applications ELISA,WB,IHC-P,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 12-28 of human Apaf1. This sequence is identical in human and mouse.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes full-length mouse and human Apaf1 (115-130kDa).
Target Name Apoptotic protease-activating factor 1
Target ID Apaf1 (NT)
Uniprot ID O14727
Alternative Names APAF-1
Gene Name APAF1
Gene ID 317
Accession Number NP_001151
Sequence Location Cytoplasm
Biological Function Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis. {PubMed:10393175, PubMed:12804598}.
Research Areas Apoptosis
Background The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease- activating factor 1). Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 is ubiquitously expressed in human tissues.
Application Images
Description Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 ug/ml.
Description Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 ug/mL.
Description Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL

Immunohistochemistry: use at 1-2ug/mL

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.

Positive control: Whole cell lysate of HeLa or K562 cells.
References & Data Sheet