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Anti-Annexin A1 Antibody (90002)

$520.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IP Human, Bovine
SKU: 90002 Categories: , ,
Overview
Product Name Anti-Annexin A1 Antibody (90002)
Description Anti-Annexin A1 Mouse Monoclonal Antibody
Target Annexin A1
Species Reactivity Human, Bovine
Applications WB,IP
Host Mouse
Clonality Monoclonal
Clone ID A1A7
Isotype IgG2b
Immunogen Annexin 1 isolated from human neutrophils.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes human and bovine Annexin 1.
Target Name Annexin A1
Target ID Annexin A1
Uniprot ID P04083
Alternative Names Annexin I, Annexin-1, Calpactin II, Calpactin-2, Chromobindin-9, Lipocortin I, Phospholipase A2 inhibitory protein, p35 [Cleaved into: Annexin Ac2-26]
Gene Name ANXA1
Sequence Location Nucleus, Cytoplasm, Cell projection, cilium, Cell membrane, Membrane, Endosome membrane, Basolateral cell membrane, Apical cell membrane, Lateral cell membrane, Secreted, Secreted, extracellular space, Cell membrane, Secreted, extracellular exosome, Cytoplasmic vesicle, secretory vesicle lumen, Cell projection, phagocytic cup, Early endosome, Cytoplasmic vesicle membrane
Biological Function Plays important roles in the innate immune response as effector of glucocorticoid-mediated responses and regulator of the inflammatory process. Has anti-inflammatory activity (PubMed:8425544). Plays a role in glucocorticoid-mediated down-regulation of the early phase of the inflammatory response (By similarity). Contributes to the adaptive immune response by enhancing signaling cascades that are triggered by T-cell activation, regulates differentiation and proliferation of activated T-cells (PubMed:17008549). Promotes the differentiation of T-cells into Th1 cells and negatively regulates differentiation into Th2 cells (PubMed:17008549). Has no effect on unstimulated T cells (PubMed:17008549). Negatively regulates hormone exocytosis via activation of the formyl peptide receptors and reorganization of the actin cytoskeleton (PubMed:19625660). Has high affinity for Ca(2+) and can bind up to eight Ca(2+) ions (By similarity). Displays Ca(2+)-dependent binding to phospholipid membranes (PubMed:2532504, PubMed:8557678). Plays a role in the formation of phagocytic cups and phagosomes. Plays a role in phagocytosis by mediating the Ca(2+)-dependent interaction between phagosomes and the actin cytoskeleton (By similarity). {UniProtKB:P10107, UniProtKB:P19619, PubMed:17008549, PubMed:19625660, PubMed:2532504, PubMed:2936963, PubMed:8425544, PubMed:8557678}.; [Annexin Ac2-26]: Functions at least in part by activating the formyl peptide receptors and downstream signaling cascades (PubMed:22879591, PubMed:15187149, PubMed:25664854). Promotes chemotaxis of granulocytes and monocytes via activation of the formyl peptide receptors (PubMed:15187149). Promotes rearrangement of the actin cytoskeleton, cell polarization and cell migration (PubMed:15187149). Promotes resolution of inflammation and wound healing (PubMed:25664854). Acts via neutrophil N-formyl peptide receptors to enhance the release of CXCL2 (PubMed:22879591). {PubMed:15187149, PubMed:22879591, PubMed:25664854}.
Research Areas Apoptosis
Background Annexin 1 belongs to a family of Ca(2+)- dependent phospholipid binding proteins which have molecular weights of approximately 35-40kDa and are localized on the cytosolic face of the plasma membrane. Annexin 1, an endogenous anti-inflammatory protein, has roles in many diverse cellular functions, such as membrane aggregation, inflammation, phagocytosis, proliferation, and apoptosis. Annexin 1 also possesses phosphorylation sites for important proliferative signaling molecules e.g., EGF receptor tyrosine kinase and PKC. This suggests that Annexin 1 may have a role in certain signaling pathways important in cancer. However, the exact mechanisms through which Annexin 1 exerts some or all of its effects are still not clearly understood, and may reveal important functions of Annexin 1 in tumorigenesis and cancer development.
Application Images
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions Immunoblotting: use at 1-10ug/ml. A band of ~39kDa is detected.

Immunohistochemistry: use at 2- 20ug/ml.

Immunoprecipitation: use at 1-2ug per 100-500ug of total protein.

These are recommended concentrations.

End user should determine optimal concentrations for their applications.
References & Data Sheet