Anti-Angiotensin Converting Enzyme 2 (ACE2) (19502)

$431.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human, Mouse, rat
SKU: 19502 Categories: , ,
Overview
Product Name Anti-Angiotensin Converting Enzyme 2 (ACE2) (19502)
Description Anti-Angiotensin Converting Enzyme 2 (ACE2) Rabbit Polyclonal Antibody
Target Angiotensin Converting Enzyme 2 (ACE2)
Species Reactivity Human, Mouse, rat
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Recombinant human ACE2 produced in a mammalian expression system.
Properties
Form Liquid
Concentration Lot Specific
Formulation TBS, pH 7.4, 1% BSA, 40% glycerol, 0.05% sodium azide.
Buffer Formulation Tris
Buffer pH pH 7.4
Buffer Anti-Microbial 0.05% Sodium Azide
Buffer Protein Stabilizer 1% Bovine Serum Albumin
Buffer Cryopreservative 40% Glycerol
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity Human, mouse, and rat ACE2.
Target Name Angiotensin-converting enzyme 2
Target ID Angiotensin Converting Enzyme 2 (ACE2)
Uniprot ID Q9BYF1
Gene Name ACE2
Gene ID 300335
Sequence Location [Processed angiotensin-converting enzyme 2]: Secreted, Cytoplasm, Cell projection, cilium, Apical cell membrane
Biological Function Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis (PubMed:27217402). Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II (PubMed:10969042, PubMed:10924499, PubMed:11815627, PubMed:19021774, PubMed:14504186). Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin (PubMed:10969042, PubMed:11815627). Also cleaves other biological peptides, such as apelins (apelin-13, [Pyr1]apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency (PubMed:11815627, PubMed:27217402, PubMed:28293165). In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity (PubMed:18424768, PubMed:19185582). {PubMed:10924499, PubMed:10969042, PubMed:11815627, PubMed:14504186, PubMed:18424768, PubMed:19021774, PubMed:19185582, PubMed:27217402}.; [Isoform 2]: Non-functional as a carboxypeptidase. {PubMed:33077916}.; (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. {PubMed:14647384, PubMed:15452268, PubMed:15791205, PubMed:15897467, PubMed:19901337, PubMed:24227843, PubMed:32142651, PubMed:32155444, PubMed:32221306, PubMed:32225175, PubMed:33000221, PubMed:33082294, PubMed:33432067}.; [Isoform 2]: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2. {PubMed:33077916, PubMed:33432184}.
Application Images
Description Immunoblotting: use at dilutions of 1:1,0001:5,000. A band of ~92kDa is detected. Detection of ACE2 in human kidney lysate with #19502 diluted 1:1,000.
Description IHC: use at dilutions of 1:50-1:200. Detection of ACE2 in paraffin-embedded human kidney.
Description Detection of ACE2 in paraffin-embedded mouse kidney. Detection of ACE2 in paraffin-embedded human breast carcinoma.
Description Detection of ACE2 in paraffin-embedded mouse kidney. Detection of ACE2 in paraffin-embedded mouse kidney.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilutions of 1:1,000-1:5,000. A band of ~92kDa is detected. Detection of ACE2 in human kidney lysate with #19502 diluted 1:1,000

IHC: use at dilutions of 1:50-1:200. Detection of ACE2 in paraffin-embedded human kidney. Detection of ACE2 in paraffin-embedded human breast carcinoma. Detection of ACE2 in paraffin-embedded mouse kidney

ICC: use at dilutions of 1:100-1:500. Detection of ACE2 in MCF-7 cells (green) after paraformaldehyde fixation and permeabilization with 0.25% Triton X100 in PBS. Detection of ACE2 in HepG2 cells (green) after paraformaldehyde fixation and permeabilization with 0.25% Triton X100 in PBS

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet