Anti-6 His Antibody, HRP conjugate (18814P)

$522.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IHC,ICC,ELISA 6 His
SKU: 18814P Categories: , ,
Overview
Product Name Anti-6 His Antibody, HRP conjugate (18814P)
Description Anti-6 His HRP Rabbit Polyclonal Antibody
Target 6 His
Species Reactivity 6 His
Applications WB,IHC,ICC,ELISA
Host Rabbit
Clonality Polyclonal
Isotype Whole IgG
Conjugate HRP
Conjugate Ratio HRP, Molar enzyme/antibody ratio is 4:1.
Immunogen 6-His (HHHHHH) conjugated to KLH.
Source Escherichia coli
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Phosphate Buffered Saline (PBS) containing 0.2% BSA and 0.05% Pro-Clean 400
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.1% Pro-Clean 400
Buffer Protein Stabilizer 0.2% Bovine Serum Albumin
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Purity Greater than 95% as determined by SDS-PAGE and RP- HPLC
Specificity Information
Specificity Antibody was affinity-purified on the peptide. This antibody does not recognize all N-terminal 6-His tagged proteins.
Target ID 6 His
Research Areas Epitope Tags
Background A polyhistidine-tag consists of at least six histidine (His) residues, often at the N- or C-terminus of a protein. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.
Application Images
Description Detection of 6-His-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity-purified, HRP-conjugated, rabbit anti-6-His antibody 18814P used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 3 seconds.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at 2-8°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:1,000-1:30,000

ELISA: use at a dilution of 1:10,000-1:100,000.

Immunocytochemistry: use at a dilution of 1:200-1:500

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet