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Anti-Gingipain B (RgpB) Antibody (13925)

$394.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug IHC, IB Poryphyromonas gingivalis
SKU: 13925 Categories: , ,
Overview
Product Name Anti-Gingipain B (RgpB) Antibody (13925)
Description Anti-Gingipain B (RgpB) Mouse Monoclonal Antibody
Target Gingipain B
Species Reactivity Poryphyromonas gingivalis
Applications IHC, IB
Host Mouse
Clonality Monoclonal
Clone ID 18E6.F7
Isotype IgG1
Immunogen Arginine-gingipain B (RgpB) from Porphyromonas gingivalis.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes a unique epitope within the immunoglobulin (Ig)-like domain of arginine-gingipain (RgpB).
Target Name Gingipain B
Target ID Gingipain B
Uniprot ID Q51844
Alternative Names Arginine-gingipain B
Gene Name PORGN
Background Arginine-gingipain B (RgpB) is a cysteine protease produced exclusively by Porphyromonas gingivalis, and it plays a central role in the pathogenicity of P. gingivalis in periodontal disease. Over the last several years, a possible association between Alzheimer disease (AD) and chronic periodontitis has been suggested based on detection of P. gingivalis infiltration in autopsy specimens from the brains of people with AD in association with neurons, tau tangles, and beta-amyloid as well as in cerebrospinal fluid of individuals diagnosed with AD. Oral administration of a gingipain inhibitor to mice with established brain infection by P. gingivalis decreased the amount of P. gingivalis DNA in brain as well as the levels of beta-amyloid and tumor necrosis factor-α. Taken together, the data suggest that gingipain inhibitors might be an effective treatment of AD. Clinical trials of gingipain inhibitors are currently underway.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Paraffin-embedded formalin-fixed AD hippocampus and gingival tissue from a patient with periodontal disease stained with #13925 and compared to non-AD control tissue and mouse IgG1 control (see Dominy et al. Science Advances 5 (1): eaau3333).
Detection of RgpB in P. gingivalis wild-type strain W83 whole cells (see Nguyen K-A et al. J Bacteriol 189: 833-843).
References & Data Sheet
References Dominy S et al. 2019. Porphyromonas gingivalis in Alzheimer’s disease brains: Evidence for disease causation and treatment with small-molecule inhibitors. Science Advances 5 (1): eaau3333. Nguyen K-A et al. 2007. Does the importance of the C-terminal residues in the maturation of RgpB from Porphyromonas gingivalis reveal a novel mechanism for protein export in a subgroup of Gram-negative bacteria? J Bacteriol 189: 833-843.