Anti-Human pThr474 RIPK2 Rabbit Monoclonal Antibody (19510)

$495.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB,IHC Human, Mouse
SKU: 19510 Categories: , , Tag:
Overview
Product Name Anti-Human pThr474 RIPK2 Rabbit Monoclonal Antibody (19510)
Description Anti-Human pThr474 RIPK2 Rabbit Monoclonal Antibody
Target RIPK2 (phospho-Thr474)
Species Reactivity Human, Mouse
Applications WB,IHC
Host Rabbit
Clonality Polyclonal
Immunogen Phosphopeptide corresponding to the pY474 site of human RIPK2.
Properties
Form Liquid
Concentration Lot Specific
Formulation Whole antiserum
Buffer Formulation Whole Antiserum
Format Whole Antiserum
Purification Whole antiserum
Specificity Information
Specificity Human and mouse RIPK2.
Target Name Receptor-interacting serine/threonine-protein kinase 2
Target ID RIPK2 (phospho-Thr474)
Uniprot ID O43353
Gene Name RIPK2
Gene ID 603455
Post Translational Modification RIPK2 (phospho-Thr474)
Target pThr474
Sequence Location Cytoplasm
Biological Function Serine/threonine/tyrosine kinase that plays an essential role in modulation of innate and adaptive immune responses. Upon stimulation by bacterial peptidoglycans, NOD1 and NOD2 are activated, oligomerize and recruit RIPK2 through CARD-CARD domains. Contributes to the tyrosine phosphorylation of the guanine exchange factor ARHGEF2 through Src tyrosine kinase leading to NF-kappaB activation by NOD2. Once recruited, RIPK2 autophosphorylates and undergoes 'Lys-63'-linked polyubiquitination by E3 ubiquitin ligases XIAP, BIRC2 and BIRC3. The polyubiquitinated protein mediates the recruitment of MAP3K7/TAK1 to IKBKG/NEMO and induces 'Lys-63'-linked polyubiquitination of IKBKG/NEMO and subsequent activation of IKBKB/IKKB. In turn, NF-kappa-B is released from NF-kappa-B inhibitors and translocates into the nucleus where it activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Plays also a role during engagement of the T-cell receptor (TCR) in promoting BCL10 phosphorylation and subsequent NF-kappa-B activation. Plays a role in the inactivation of RHOA in response to NGFR signaling (PubMed:26646181). {PubMed:14638696, PubMed:17054981, PubMed:18079694, PubMed:21123652, PubMed:21887730, PubMed:26646181}.
Application Images
Description Immunoblotting: use at a dilution of 1:500. Detection of RIPK2 in MDP-stimulated HEK293 cells (+) vs. non-stimulated HEK293 cells (-).
Description Immunohistochemistry: use at a dilution of 1:100. Detection of active RIPK2 in human (top) and mouse (bottom) tissues. Non-IBD refers to colon sections from non-inflammatory bowel disease (IBD) patients; IBD refers to colon sections from ulcerative colitis (UC) patients. Mouse strain used: B6.129P2-I110tm1Cgn/J, a common model for IBD that presents with spontaneous colitis that is elevated by DSS (dextran sulfate sodium) exposure.
Additional Information
Handling
Storage Store at -20°C in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at a dilution of 1:500. Detection of RIPK2 in MDP-stimulated HEK293 cells (+) vs. non-stimulated HEK293 cells (-)

These are recommended dilutions.

Endusers should determine optimal dilutions for their applications.

Immunohistochemistry: use at a dilution of 1:100. Detection of active RIPK2 in human (top) and mouse (bottom) tissues. Non-IBD refers to colon sections from non-inflammatory bowel disease (IBD) patients; IBD refers to colon sections from ulcerative colitis (UC) patients. Mouse strain used: B6.129P2-I110tm1Cgn/J, a common model for IBD that presents with spontaneous colitis that is elevated by DSS (dextran sulfate sodium) exposure.
References & Data Sheet