Anti-Bassoon Antibody (56495)

$435.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,IHC,IF Human, Mouse, Rat
SKU: 56495 Categories: , ,
Overview
Product Name Anti-Bassoon Antibody (56495)
Description Anti-Bassoon Rabbit Polyclonal Antibody
Target Bassoon
Species Reactivity Human, Mouse, Rat
Applications WB,IHC,IF
Host Rabbit
Clonality Polyclonal
Immunogen Fusion protein corresponding to aa786-1041 (N-terminal) of human Bassoon (accession no. NM_003458.3).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Bassoon. Multiple isoforms are detected.
Target Name Protein bassoon
Target ID Bassoon
Uniprot ID Q9UPA5
Alternative Names Zinc finger protein 231
Gene Name BSN
Sequence Location Cytoplasm, Cell junction, synapse, presynaptic active zone, Cytoplasm, cytoskeleton, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane
Biological Function Scaffold protein of the presynaptic cytomatrix at the active zone (CAZ) which is the place in the synapse where neurotransmitter is released (PubMed:12812759). After synthesis, participates in the formation of Golgi-derived membranous organelles termed Piccolo-Bassoon transport vesicles (PTVs) that are transported along axons to sites of nascent synaptic contacts (PubMed:19380881). At the presynaptic active zone, regulates the spatial organization of synaptic vesicle cluster, the protein complexes that execute membrane fusion and compensatory endocytosis (By similarity). Functions also in processes other than assembly such as the regulation of specific presynaptic protein ubiquitination by interacting with SIAH1 or the regulation of presynaptic autophagy by associating with ATG5 (By similarity). Mediates also synapse to nucleus communication leading to rUniProtKB:O88778, PubMed:12812759, PubMed:19380881}.
Research Areas Neuroscience
Background Bassoon, a 420kDa protein, is localized at presynaptic nerve terminals and appears to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The C-terminal regions of the polyglutamine domains are of interest since abnormal amplification of this region in some human proteins, such as Huntingtin, can cause late-onset neuro- degeneration.
Application Images
Description Immunoblotting: use at 1ug/ml. Immunoblot on rat and mouse brain lysates.
Description Immunofluorescence: use at 1-10ug/ml. Bassoon, BTX, and overlay. Neuromuscular junction whole muscle samples from adult mouse with #56495 diluted 1:400 and Alexa 488conjugated secondary antibody.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Immunohistochemistry: use at 1-10ug/mL.

Immunofluorescence: use at 1-10ug/mL.

Positive control: Rat brain tissue lysate

These are recommended concentrations;

Enduser should determine optimal concentrations for their applications.
References & Data Sheet
References Garton AJ et al. 1996 Mol Cell Biol 16: 6408-6418.
Garton AJ and Tonks NK 1999 J Biol Chem 274: 3811-3818.