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Anti-Mouse Sperm Protein sp56 Antibody (55102)

$382.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA,IHC Mouse, Hamster
SKU: 55102 Categories: , ,
Overview
Product Name Anti-Mouse Sperm Protein sp56 Antibody (55102)
Description Anti-Mouse Sperm Protein sp56 Mouse Monoclonal Antibody
Target Mouse Sperm Protein sp56
Species Reactivity Mouse, Hamster
Applications WB,ELISA,IHC
Host Mouse
Clonality Monoclonal
Clone ID 5F12
Isotype IgG1
Immunogen Mixture of native and formaldehyde-zinc fixed mouse sperm purified through a Percoll gradient
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity These antibodies specifically recognize mouse sperm protein sp56. They will react with native or denatured, non-reduced sp56 but not with denatured, reduced sp56. These antibodies do not cross-react with human sperm. Relative antibody titers for antibody binding to solid phase sp56: 55101 = 66pM, 55102 = 4nM, 55103 = 29nM, 55104 = 70nM
Target ID Mouse Sperm Protein sp56
Research Areas Reproductive Biology
Application Images
Additional Information
Handling
Storage These antibodies are stable for at least on (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions These antibodies may be used in

Immunohistochemistry on zinc-formaldehyde fixed, paraffin-embedded mouse testes to localize sp56 on mouse sperm and in immunoblots or ELISA to detect sp56.
References & Data Sheet
References Cheng A. et al. 1994. Sperm-Egg Recognition in the Mouse: Characterization of sp56, A Sperm Protein Having Specific Affinity for ZP3. J Cell Biol 125: 867-878.
Bookbinder LH et al. 1995. Tissue- and Species-Specific Expression of sp56, a Mouse Sperm Fertilization Protein. Science 269: 86-89.