Anti-Beclin 2 Antibody (23011)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ICC/IF Human, Rat
SKU: 23011 Categories: , ,
Overview
Product Name Anti-Beclin 2 Antibody (23011)
Description Anti-Beclin2 Rabbit Polyclonal Antibody
Target Beclin 2
Species Reactivity Human, Rat
Applications WB,ICC/IF
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to mid-protein amino acids of human Beclin 2.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Concentration: 1mg/ml
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity This antibody recognizes human and rat Beclin 2.
Target Name Beclin-2
Target ID Beclin 2
Uniprot ID A8MW95
Alternative Names Beclin-1 autophagy-related pseudogene 1, Beclin-1-like protein 1
Gene Name BECN2
Sequence Location Cytoplasm
Biological Function Involved in 2 distinct lysosomal degradation pathways: acts as a regulator of autophagy and as a regulator of G-protein coupled receptors turnover. Regulates degradation in lysosomes of a variety of G-protein coupled receptors via its interaction with GPRASP1/GASP1. {PubMed:23954414}.
Research Areas Autophagy
Background Autophagy is a catabolic process that results in the degradation of bulk cytoplasmic contents within autophagosomes and lysosomes. Beclin 2 is an Atg6/Beclin family member that functions in two distinct lysosomal degradation pathways, autophagy and the downregulation of certain seven- transmembrane-spanning GPCRs. Its role in GPCR lysosomal degradation does not involve Beclin 1 or other Beclin 1/2-interacting proteins such as ATG14 and VPS34 that function in autophagy. Its role in lysosomal degradation also appears to differ from other autophagy- independent functions previously described for yeast Atg6/Vps30 or mammalian Beclin 1, such as vacuolar protein sorting and EGFR degradation.
Application Images
Description Immunoblotting: use at 1-2ug/ml. A band of ~50kDa is detected. Detection of Beclin 2 in 20ug rat liver lysate.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-2ug/mL. A band of ~50kDa is detected.Detection of Beclin 2 in 20ug rat liver lysate.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet