Anti-GPR15/BOB Antibody (2101)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC Human, Mouse, Rat
SKU: 2101 Categories: , ,
Overview
Product Name Anti-GPR15/BOB Antibody (2101)
Description Anti-GPR15 (NT) Rabbit Polyclonal Antibody
Target GPR15/BOB
Species Reactivity Human, Mouse, Rat
Applications ELISA,WB,ICC
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 13- 28 of human GPR15. This sequence differs from those of African green monkey and pig-tailed macaque BOB by one amino acid.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes GPR15/BOB of human, mouse, and rat (50 kD).
Target Name G-protein coupled receptor 15
Target ID GPR15/BOB
Uniprot ID P49685
Alternative Names Brother of Bonzo, BoB
Gene Name GPR15
Gene ID 2838
Accession Number NP_005281
Sequence Location Cell membrane; Multi-pass membrane protein.
Biological Function Probable chemokine receptor. Alternative coreceptor with CD4 for HIV-1 infection.
Research Areas Infectious Disease
Background Human immunodeficiency virus (HIV) and related viruses require co-receptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1, and V28 have been recently identified as HIV co-receptors. The genes encoding human and monkey GPR15/BOB (for G protein- coupled receptor 15 and brother of Bonzo) have been cloned. This novel g protein- coupled receptor serves as co-receptor for SIV and for strains of HIV-2 and M-tropic HIV-1. The ligand for GPR15 is under investigation.
Application Images
Description Western blot analysis of GPR15 in human spleen lysate with GPR15 antibody at (A) 0.5 and (B) 1 ug/mL.
Description Immunocytochemistry of GPR15 in A431 cells with GPR15 antibody at 5 ug/ml.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500-1:2,000 dilution.

Positive control: Jurkat or A549 cell lysate.
References & Data Sheet