Anti-Endothelin Antibody (19402)

$482.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug ELISA,WB,IHC Human
SKU: 19402 Categories: , ,
Overview
Product Name Anti-Endothelin Antibody (19402)
Description Anti-Endothelin Mouse Monoclonal Antibody
Target Endothelin
Species Reactivity Human
Applications ELISA,WB,IHC
Host Mouse
Clonality Monoclonal
Clone ID ENDO20-2111.41
Isotype IgG1
Immunogen Human endothelin-1 (ET-1)
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity Human endothelin-1 (ET-1).
Target Name Endothelin-1
Target ID Endothelin
Uniprot ID P05305
Alternative Names Preproendothelin-1, PPET1 [Cleaved into: Endothelin-1
Gene Name EDN1
Sequence Location Secreted.
Biological Function Endothelins are endothelium-derived vasoconstrictor peptides (By similarity). Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells (PubMed:19086031). Also binds the DEAR/FBXW7-AS1 receptor (PubMed:17446437). {UniProtKB:P09558, PubMed:17446437, PubMed:19086031}.
Research Areas Hormones/Proteins
Application Images
Description Activity and specificity
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions

Immunohistochemistry:Paraffin-embedded rat kidney exposed to 60 minutes of ischemia. ET-1 expression is seen in the endothelium of the peritubular capillary network (arrows, dark reaction product) with #19401 at 5-20ug/ml. Wilhelm SM et al. 1999 Kidney International 55: 1011-1018.ELISA:use at 5-20ug/ml with endothelin-1 on the solid phase.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet