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Mn Superoxide Dismutase (13003P-25)

$287.00

Host Quantity Applications Species Reactivity Data Sheet  
25ug WB Human
SKU: 13003P-25 Categories: ,
Overview
Product Name Mn Superoxide Dismutase (13003P-25)
Description Recombinant Human Mn SOD2 produced in E. coli is a single, non-glycosylated polypeptide chain containing 219 amino acidsaa 25-222) with a molecular weight of 24.4kDa. It has a His- tag at the N-terminus.
Target Mn Superoxide Dismutase
Species Reactivity Human
Applications WB
Source Escherichia coli
Properties
Form Liquid
Concentration Lot Specific
Formulation Sterile-filtered solution in 20mM Tris-HCl, pH 8.0, 20% glycerol. 
Buffer Formulation 20 mM Tris
Buffer pH pH 8.0
Buffer Cryopreservative 20% glycerol
Purity Greater than 95% as determined by SDS- PAGE
Background SOD2 is part of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. SOD2 binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in SOD2 gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. SOD2 destroys radicals which are usually produced within the cells and which are toxic to biological systems.
Specificity Information
Target Name Superoxide dismutase [Mn], mitochondrial
Target ID Mn Superoxide Dismutase
Alternative Names rHuMnSOD (SOD2)
Sequence Location Mitochondrion matrix.
Sequence MGSSHHHHHH SSGLVPRGSH MKHSLPDLPY DYGALEPHIN AQIMQLHHSK HHAAYVNNLN VTEEKYQEAL AKGDVTAQIA LQPALKFNGG GHINHSIFWT NLSPNGGGEP KGELLEAIKR DFGSFDKFKE KLTAASVGVQ GSGWGWLGFN KERGHLQIAA CPNQDPLQGT TGLIPLLGID VWEHAYYLQY KNVRPDYLKA IWNVINWENV TERYMACKK.
Biological Activity Mn Superoxide Dismutase
Biological Function Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems. {PubMed:10334867}.
Background SOD2 is part of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. SOD2 binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in SOD2 gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. SOD2 destroys radicals which are usually produced within the cells and which are toxic to biological systems.
Application Images
Additional Information
Handling
Storage Store at 4°C if entire vial will be used within 2-4 weeks. Store at -20°C for longer periods of time. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
References & Data Sheet