Anti-Eotaxin Antibody (1123)

$445.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug ELISA,WB,ICC,IF Human
SKU: 1123 Categories: , ,
Overview
Product Name Anti-Eotaxin Antibody (1123)
Description Anti-Eotaxin (CT) Rabbit Polyclonal Antibody
Target Eotaxin
Species Reactivity Human
Applications ELISA,WB,ICC,IF
Host Rabbit
Clonality Polyclonal
Isotype IgG
Immunogen Peptide corresponding to aa 83-97 of human eotaxin.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes the monomeric (9 kD) and dimeric (18 kD) forms of human eotaxin
Target Name Eotaxin
Target ID Eotaxin
Uniprot ID P51671
Alternative Names C-C motif chemokine 11, Eosinophil chemotactic protein, Small-inducible cytokine A11
Gene Name CCL11
Gene ID 6356
Accession Number NP_002977.1
Sequence Location Secreted
Biological Function In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions (PubMed:8597956). Binds to CCR3 (PubMed:8631813). {PubMed:8597956, PubMed:8631813}.
Research Areas Infectious Disease
Background Chemokines play a key role in inflammation. The CC chemokine eotaxin is a potent and specific eosinophil chemoattractant that is expressed by a variety of cell types in certain inflammatory conditions. Some G-protein coupled chemokine receptors are also utilized as virus coreceptors for fusion and infection of cells. The eotaxin receptor CCR3 is required for HIV-1 entry into target cells such as microglia, and eotaxin inhibits infection by HIV-1.
Application Images
Description Western blot analysis of eotaxin in HeLa cell lysate containing 10, 2.5, or 1 ng of full length recombinant eotaxin with eotaxin antibody at 1 ug/mL.
Description Immunocytochemistry of Eotaxin in 293 cells with Eotaxin antibody at 5 ug/mL.
Description Immunofluorescence of Eotaxin in 293 cells with Eotaxin antibody at 10 ug/mL.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution.

Positive control: Native and recombi- nant protein.
References & Data Sheet