Anti-Grp94 Antibody (11097)

$338.00

Host Quantity Applications Species Reactivity Data Sheet  
Rat 100ug WB,ICC/IF,IP,FCM Human, Mouse, Rat, Rabbit, Monkey, Hamster, Guinea Pig, Dog, Bovine, Sheep, Pig, Chicken, Xenopus
SKU: 11097 Categories: , ,
Overview
Product Name Anti-Grp94 Antibody (11097)
Description Anti-Grp94 Rat Monoclonal Antibody
Target Grp94
Species Reactivity Human, Mouse, Rat, Rabbit, Monkey, Hamster, Guinea Pig, Dog, Bovine, Sheep, Pig, Chicken, Xenopus
Applications WB,ICC/IF,IP,FCM
Host Rat
Clonality Monoclonal
Clone ID 9G10
Isotype IgG2a
Immunogen Purified Grp94 isolated from chicken oviducts.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes an ~98 kDa protein corresponding to the predicted molecular mass of Grp94 on SDS-PAGE immunoblots in human, mouse, rat, rabbit, monkey, hamster, guinea pig, dog, bovine, sheep, pig, chicken and Xenopus.
Target Name Endoplasmin
Target ID Grp94
Uniprot ID P14625
Alternative Names 94 kDa glucose-regulated protein, GRP-94, Heat shock protein 90 kDa β member 1, Tumor rejection antigen 1, gp96 homolog
Gene Name HSP90B1
Gene ID 7184
Accession Number NP_003290.1
Sequence Location Endoplasmic reticulum lumen, Sarcoplasmic reticulum lumen, Melanosome
Biological Function Molecular chaperone that functions in the processing and transport of secreted proteins (By similarity). When associated with CNPY3, required for proper folding of Toll-like receptors (By similarity). Functions in endoplasmic reticulum associated degradation (ERAD) (PubMed:18264092). Has ATPase activity (By similarity). May participate in the unfolding of cytosolic leaderless cargos (lacking the secretion signal sequence) such as the interleukin 1/IL-1 to facilitate their translocation into the ERGIC (endoplasmic reticulum-Golgi intermediate compartment) and secretion; the translocation process is mediated by the cargo receptor TMED10 (PubMed:32272059). {UniProtKB:P08113, PubMed:18264092, PubMed:32272059}.
Research Areas Heat Shock& Stress Proteins
Background Glucose-regulated protein 94 (Grp94) is an endoplasmic reticulum luminal stress protein which belongs to the Hsp90 family of molecular chaperones. The sequence of Grp94 includes the COOH-terminal tetrapeptide Lys-Asp-Glu-Leu (KDEL) which is a signal sequence that causes proteins to be retained in the pre-Golgi compartments. Expression of Grp94 is up-regulated by stress conditions such as glucose starvation and heat shock, and it functions in intracellular trafficking of peptides from the extracellular space to the MHC class I antigen processing pathway of antigen- presenting cells.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (11097). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (11097) at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rat (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Description Immunocytochemistry/Immunofluorescence analysis using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (11097). Tissue: Heat Shocked cervical cancer cells (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (11097) at 1:100 for 12 hours at 4°C. Secondary Antibody: APC Goat Anti-Rat (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-GRP94 Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
Description Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of GRP94 protein using Rat Anti-GRP94 Monoclonal Antibody, Clone 9G10 (11097). Primary Antibody: Rat Anti-GRP94 Monoclonal Antibody (11097) at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1ug/mL. A band of ~98 kDa is detected.

Immunohistochemistry: use at 2-30ug/mL.

Immunoprecipitation: 10ug/mL.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: HeLa cell lysate.
References & Data Sheet