Anti-KDEL Receptor Antibody (11078)

$447.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,IP Human, Monkey, Mouse, Rat, Hamster, Rabbit, Porcine, Bovine, Ovine, Canine, Chicken, Drosophila, Xenopus
SKU: 11078 Categories: , ,
Overview
Product Name Anti-KDEL Receptor Antibody (11078)
Description Anti-KDEL Receptor Mouse Monoclonal Antibody
Target KDEL Receptor
Species Reactivity Human, Monkey, Mouse, Rat, Hamster, Rabbit, Porcine, Bovine, Ovine, Canine, Chicken, Drosophila, Xenopus
Applications WB,IHC,ICC/IF,IP
Host Mouse
Clonality Monoclonal
Clone ID KR-10
Isotype IgG1
Immunogen Peptide corresponding to aa 192-212 of bovine KDEL receptor
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.2, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.2
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Target Name ER lumen protein-retaining receptor 1
Target ID KDEL Receptor
Uniprot ID Q99JH8
Alternative Names KDEL endoplasmic reticulum protein retention receptor 1, KDEL receptor 1
Gene Name Kdelr1
Gene ID 68137
Accession Number NP_598711.1
Sequence Location Golgi apparatus membrane, Cytoplasmic vesicle, COPI-coated vesicle membrane, Endoplasmic reticulum membrane, Endoplasmic reticulum-Golgi intermediate compartment membrane
Biological Function Receptor for the C-terminal sequence motif K-D-E-L that is present on endoplasmic reticulum resident proteins and that mediates their recycling from the Golgi back to the endoplasmic reticulum. {UniProtKB:P24390}.
Research Areas Neuroscience
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 (11078). Tissue: NRK cells. Species: Rat. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody (11078) at 1:1000. Secondary Antibody: APC Goat Anti-Mouse (red). Counterstain: DAPI (blue) nuclear stain. KR-10 staining red; DAPI staining blue. Merged images. Courtesy of: Institute of Mol. and Cell Bio, Singapore.
Description Western Blot analysis of Rat tissue lysate showing detection of KDEL Receptor protein using Mouse Anti-KDEL Receptor Monoclonal Antibody, Clone KR-10 (11078). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KDEL Receptor Monoclonal Antibody (11078) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Western blot: use at 1ug/ml. A band of approx. 25kDA is detected. Detection of KDEL receptor in rat tissue lysate with #11078 at 1ug/ml.
References & Data Sheet