Awaiting product image

Anti-Bcl-3 Antibody (11067)

$377.00

Host Quantity Applications Species Reactivity Data Sheet  
Hamster 100ug WB,ELISA Human, Mouse
SKU: 11067 Categories: , ,
Overview
Product Name Anti-Bcl-3 Antibody (11067)
Description Anti-Bcl-3 Hamster Monoclonal Antibody
Target Bcl-3
Species Reactivity Human, Mouse
Applications WB,ELISA
Host Hamster
Clonality Monoclonal
Clone ID 150-3.5
Isotype IgG1
Immunogen Recombinant mouse Bcl-3- 6His.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse Bcl-3.
Target Name B-cell lymphoma 3 protein
Target ID Bcl-3
Uniprot ID P20749
Alternative Names BCL-3, Proto-oncogene BCL3
Gene Name BCL3
Gene ID 602
Accession Number NP_005169
Sequence Location Nucleus. Cytoplasm, Cytoplasm, perinuclear region
Biological Function Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation (By similarity). {PubMed:8453667}.
Research Areas Apoptosis
Background The gene encoding Bcl-3 was first identified as the gene altered in some cases of chronic B cell lymphocytic leukemia and was later found to belong to the IkappaB subfamily of inhibitors of NFkappaB transcription factors. Bcl- 3 does not have DNA-binding activity, but it interacts with NFkappaB1 and NFkappaB2 which, when bound to DNA, repress gene transcription. Therefore, Bcl-3 may promote gene expression that is repressed by members of the NFkappaB family. Bcl-3 RNA is highly expressed in spleen and liver with lower levels of expression in heart, skeletal muscle, lung, kidney, and testes.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 2ug/ml. A band of approx. 64kDa is detected

ELISA:

End user should determine optimal concentration for each sample.

Positive control: Jurkat T cell lysate.See Bassetti MFJ et al. 2009 International Immunology 21: 339-348.
References & Data Sheet