Anti-Bacterial Transglutaminase Antibody (90003)

$520.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA Streptomyces
SKU: 90003 Categories: , ,
Overview
Product Name Anti-Bacterial Transglutaminase Antibody (90003)
Description Anti-Bacterial Transglutaminase Mouse Monoclonal Antibody
Target Bacterial Transglutaminase
Species Reactivity Streptomyces
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 3C7
Isotype IgG1
Immunogen Recombinant Streptomyces mobaraensis transglutaminase.
Properties
Form Lyophilized
Formulation Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose.
Buffer Formulation Tris Buffer
Format Purified
Purification Purified by Protein A affinity chromatography
Specificity Information
Specificity Antigen: Recombinant Streptomyces mobaraensis transglutaminase.
Target Name Protein-glutamine γ-glutamyltransferase
Target ID Bacterial Transglutaminase
Uniprot ID P81453
Alternative Names EC 2.3.2.13, MTG, Transglutaminase, TGase
Biological Function Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Research Areas Enzymes
Background Transglutaminases (Tgases) are multifunctional enzymes that are widely distributed among mammals, inverte- brates and plants. Streptomyces mobaraensis Tgase is one of the most useful Tgases due to its rather broad substrate specificity and independence of Ca2+. The role of TGase in the life- cycle of Streptomycetes is not fully understood, but it is likely that it functions in protein cross-linking that fortifies the aerial cell wall and the spore envelope. The protein structures of the known bacterial Tgases are quite different from mammalian Tgases in that they lack sequence homology and have smaller molecular masses. Tgase from S. mobaraensis is secreted as a 42.5- kDa precursor protein which is then processed by the removal of a 45 amino-acid N-terminal peptide.
Application Images
Description Immunoblotting: use at 1-10ug/ml. A band of ~38kDa is detected.
Additional Information
Handling
Storage This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Dilution Instructions Reconstitute lyophilized antibody using ultrapure to a final concentration no less than 100 ug/mL. Reconstitution with PBS or Tris buffer is acceptable if required. Protein carrier, biocide or cryopreservative can be added as needed. Aliquot and store at -20C for longterm storage. Dilute immediately prior to use.
Application Instructions Immunoblotting: use at 1-10ug/mL. A band of ~38kDa is detected

ELISA: use at 1-5ug/mL with Streptomyces Tgase on the solid phase.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
References & Data Sheet