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CCL28, biotinylated (50183PB-2)

$153.00

Host Quantity Applications Species Reactivity Data Sheet  
2ug Migration assay Human
SKU: 50183PB-2 Categories: ,
Overview
Product Name CCL28, biotinylated (50183PB-2)
Description Recombinant human CCL28, produced in E. coli, corresponds to aa 23- 127
Target CCL28ylated
Species Reactivity Human
Applications Migration assay
Conjugate Biotin
Source Recombinant human CCL28, produced in E. coli
Properties
Form Lyophilized
Molecular Mass 14.3 kDa
Purity >97%
Background CCL28, also known as Mucosae-associated Epithelial Chemokine (MEC), is secreted by gastrointestinal and non-intestinal mucosal cells. It regulates chemotaxis of cells expressing surface receptors CCR3 and CCR10. This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
Specificity Information
Target Name C-C motif chemokine 28
Target ID CCL28ylated
Alternative Names rHuCCL28-biotin, MEC
Sequence Location Secreted.
Sequence ILPIASSCCTEVSHHISRRLLERVNMCRIQRADGDCDLAAVILHVKRRR ICVSPHNHTVKQWMKVQAAKKNGKGNVCHRKKHHGKRNSNRAHQG KHETYGHKTPY
Biological Activity CCL28ylated
Biological Function Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.
Background CCL28, also known as Mucosae-associated Epithelial Chemokine (MEC), is secreted by gastrointestinal and non-intestinal mucosal cells. It regulates chemotaxis of cells expressing surface receptors CCR3 and CCR10. This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues. It has also been shown as a potential antimicrobial agent effective against certain pathogens, such as Gram negative and Gram positive bacteria and the fungus Candida albicans.
Application Images
Additional Information
Additional Information Endotoxin Level: <0.01 EU per 1ug of protein by LAL method.Migration Assay of recombinant cells expressing CCR10.
Handling
Storage 12 months from date of receipt, -20°C to -70°C, as supplied. 1 month, -20°C to -70°C, under sterile conditions after reconstitution. Best if used immediately after reconstitution. Avoid multiple freeze-thaw cycles.
Dilution Instructions Centrifuge tube prior to resuspending. Recommended at 100ug/ml in sterile distilled water.
References & Data Sheet