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Anti-Hsp90 Antibody (11113)

$384.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA,IHC,IP Human, Mouse, Rat
SKU: 11113 Categories: , ,
Overview
Product Name Anti-Hsp90 Antibody (11113)
Description Anti-Hsp90 Mouse Monoclonal Antibody
Target Hsp90
Species Reactivity Human, Mouse, Rat
Applications WB,ELISA,IHC,IP
Host Mouse
Clonality Monoclonal
Clone ID 4F3.E8
Isotype IgG2a
Immunogen Recombinant human Hsp90 purified from E. coli.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Hsp90alpha and b. Other species have not been tested.
Target Name Heat shock protein HSP 90-α
Target ID Hsp90
Uniprot ID P07900
Alternative Names EC 3.6.4.10, Heat shock 86 kDa, HSP 86, HSP86, Lipopolysaccharide-associated protein 2, LAP-2, LPS-associated protein 2, Renal carcinoma antigen NY-REN-38
Gene Name HSP90AA1
Gene ID 3320
Accession Number NP_001017963.2
Sequence Location Nucleus, Cytoplasm, Melanosome, Cell membrane, Mitochondrion
Biological Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate rPubMed:11274138, PubMed:11276205, PubMed:12526792, PubMed:15577939, PubMed:15937123, PubMed:20628368, PubMed:24613385, PubMed:25609812, PubMed:27353360, PubMed:29127155, PubMed:25973397, PubMed:26991466, PubMed:27295069}.
Research Areas Heat Shock& Stress Proteins
Background Hsp90 is an abundantly and ubiquitously expressed heat shock protein found in all eukaryotic cells. It exists in two principal forms, alpha and beta, which share 85% sequence homology. Both forms are expressed in the cytosol. Hsp90alpha exists primarily as a homodimer while Hsp90beta exists mainly as a monomer. Hsp90 plays a role in folding, assembly, maturation, and stabilization of specific proteins as a key component of a chaperone complex. Most of the Hsp90-regulated proteins that have been identified are involved in cell signaling; kinases, v-Src, Wee1, c-Raf, and p53 are some examples. When bound to ATP, Hsp90 interacts with co- chaperones Cdc37, p23, and various immunophilin-like proteins to form complexes that stabilize and protect target proteins from proteasomal degradation.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 0.5-1ug/ml. A band of ~90 kDa is detected

User should determine optimal concentrations for their application.

Positive control: Heat-shocked HeLa cell lysate.
References & Data Sheet