Anti-Hsp70/Hsc70 (plant) Antibody (11138)

$457.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA Plant
SKU: 11138 Categories: , ,
Overview
Product Name Anti-Hsp70/Hsc70 (plant) Antibody (11138)
Description Anti-Hsp70/Hsc70 (Plant) Mouse Monoclonal Antibody
Target Hsp70/Hsc70 (plant)
Species Reactivity Plant
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 5G1-95
Isotype IgG1
Immunogen Purified Hsp70 from Phaseolus aureus (mung bean).
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes constitutive and inducible plant Hsp70. It does not cross-react with human, rat, bacteria (DNAKK) or human BIP.
Target ID Hsp70/Hsc70 (plant)
Research Areas Heat Shock& Stress Proteins
Background Hsp70 genes encode heat-inducible 70kDa heat shock proteins. Hsp70 binds ATP with high affinity and possesses weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. The structure of the ATP binding domain displays multiple features of nucleotide binding proteins. The binding of ATP triggers a critical conformational change which leads to the release of the bound substrate protein. The universal ability of Hsp70s to undergo cycles of binding to and release from partially unfolded proteins is the basis of their role in protein synthesis, protein folding, oligomerization, and protein transport.
Application Images
Description Western Blot analysis of Duckweed (Lemma minor) Heat Shocked cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone 5G1-95 (11138). Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11138) at 1:1000.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~70kDa is detected

ELISA: use at 1-10ug/mL.

These are recommended concentrations;

Enduser should determine optimal concentrations for their applications.
References & Data Sheet