Anti-Hsp70 Antibody (11096)

$338.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC,ICC/IF,ELISA,FCM,FACS,IEM,Bl,AM Human, Mouse, Rat, Rabbit, Monkey, Guinea Pig, Hamster, Chicken, Bovine, Canine, Ovine, Porcine, Fish, Drosophila, C. Elegans
SKU: 11096 Categories: , ,
Overview
Product Name Anti-Hsp70 Antibody (11096)
Description Anti-Hsp70 Mouse Monoclonal Antibody
Target Hsp70
Species Reactivity Human, Mouse, Rat, Rabbit, Monkey, Guinea Pig, Hamster, Chicken, Bovine, Canine, Ovine, Porcine, Fish, Drosophila, C. Elegans
Applications WB,IHC,ICC/IF,ELISA,FCM,FACS,IEM,Bl,AM
Host Mouse
Clonality Monoclonal
Clone ID C92F3A-5
Isotype IgG1
Immunogen Human Hsp70 purified from HeLa cells.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS containing 0.1% sodium azide
Buffer Formulation Phosphate Buffered Saline
Buffer Anti-Microbial 0.1% Sodium Azide
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes Hsp70 of human, mouse, rat, rabbit, monkey, guinea pig, hamster, chicken, bovine, canine, ovine, porcine, fish, Drosophila, and C. elegans and does not cross-react with Hsc70. The epitope is in the region of amino acids 436- 503 of human Hsp70.
Target ID Hsp70
Uniprot ID P08107
Gene ID 3303
Accession Number NP_005336.3
Research Areas Heat Shock& Stress Proteins
Background Hsp70 genes, members of a multigene family, encode heat-inducible 70kDa heat- shock proteins that function as molecular chaperones. They have been identified in most organelles of eukaryotic cells as well as in bacteria. The N-terminus of Hsp70 binds ATP with high affinity and the C- terminus binds proteins and polypeptides. Hsp70s bind nascent polypeptides as well as partially folded intermediates of proteins, thus preventing their aggregation and/or misfolding. Binding of ATP triggers a conformational change that results in the release of bound protein. Hsp70s function in protein synthesis, protein folding and oligomerization, and protein transport.
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (11096). Tissue: Heat Shocked Melanoma cells. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11096) at 1:1000 for 16 hours at RT. Secondary Antibody: Biotin Goat Anti-Mouse. Courtesy of: Dr. Ewa Malusecka, Maria Sklodowska-Curie Memorial Cancer Centre and Inst. Of Oncology, Poland.
Description Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (11096). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11096) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.
Description Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (11096). Tissue: colon carcinoma. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11096) at 1:10000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. This image was produced using an amplifying IHC wash buffer. The antibody has therefore been diluted more than is recommended for other applications.
Description Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone C92F3A-5 (11096). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody (11096) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
Description Fluorescence Activated Cell Sorting analysis using Mouse Anti-Hsp70: FITC Monoclonal Antibody, Clone C92F3A-5 (11096). Tissue: Heat Shocked CD3+ CD8+ T cells . Species: Mouse. Primary Antibody: Mouse Anti-Hsp70: FITC Monoclonal Antibody (11096) at 1:1000. Courtesy of: Cheryl Cameron, Vaccine and Gene Therapy Instit. Florida.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: 1ug/mL. A band of 70kDa is detected.Immunoblotting of Hsp70 in cell lysates from human cancer cell lines with #11096.

Immunohistochemistry: 1-10ug/mLImmunohistochemical staining of inflammatory cells in human colon tissue with #11096.

Immunoprecipitation: 1-10ug/

mLELISA: 1-10ug/mLFlow cytometry: 10ug/mL

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: HeLa cell lysate.
References & Data Sheet