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Anti-Hsp22 Antibody (11133)

$346.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB Human, Mouse, Rat
SKU: 11133 Categories: , ,
Overview
Product Name Anti-Hsp22 Antibody (11133)
Description Anti-Hsp22 Mouse Monoclonal Antibody
Target Hsp22
Species Reactivity Human, Mouse, Rat
Applications WB
Host Mouse
Clonality Monoclonal
Clone ID 3C12.H11
Isotype IgG1
Immunogen His-tagged recombinant human Hsp22
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human, mouse, and rat Hsp22. It detects endogenous and exogenous Hsp22 in monomeric, dimeric, and tetrameric (weak) forms in Western blots. Exogenous Hsp22 is detected in dimeric form.It does not cross-react with alpha crystallin.
Target Name Heat shock protein β-8, Equilibrative nucleoside transporter 1
Target ID Hsp22
Uniprot ID Q9UJY1,Q9UJY2
Gene Name HSPB8, SLC29A1
Gene ID 26353, 26354
Accession Number NP_055180.1, NP_055180.2
Research Areas Heat Shock& Stress Proteins
Background Hsp22 (HspB8) is a member of the small heat shock protein super-family. Like most other sHsps, Hsp22 is predominantly transcribed in skeletal muscle and heart as well as placenta. It is a monomeric protein which interacts with HspB1. It displays temperature- dependent chaperone activity. Hsp22 has two binding domains (N- and C- terminal) that are specific for different binding partners and has the ability to bind itself and other sHsps. Its chaperone-like activity is important to the function of Hsp22 in various processes including proliferation, apoptosis, and macroautophagy.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1-10ug/ml. A band of ~22kDa (monomeric) is detected.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: Whole rat tissue extract.
References & Data Sheet