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Anti-Grp170 Antibody (11149)

$346.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA Human
SKU: 11149 Categories: , , ,
Overview
Product Name Anti-Grp170 Antibody (11149)
Description Anti-Grp170 Mouse Monoclonal Antibody
Target Grp170
Species Reactivity Human
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 6G7-2H5
Isotype IgG2b
Immunogen Synthetic peptide sequence derived from human Grp170 (accession no. NP_006389.3)
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 0.1% sodium azide, 50% glycerol. Purified by Protein G affinity chromatography.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.1% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human Grp170 (170kDa).
Target Name Hypoxia up-regulated protein 1
Target ID Grp170
Uniprot ID Q9Y4L1
Alternative Names 150 kDa oxygen-regulated protein, ORP-150, 170 kDa glucose-regulated protein, GRP-170
Gene Name HYOU1
Accession Number NP_006389.3
Sequence Location Endoplasmic reticulum lumen.
Biological Function Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation. May play a role as a molecular chaperone and participate in protein folding. {PubMed:10037731}.
Research Areas Heat Shock& Stress Proteins
Background Grp170 is a major chaperone/stress protein resident in the ER. It is distantly related in sequence to both hsp110 and hsp70 families, but represents the Grp170 stress protein family. Studies have suggested that Grp170 is an important element of the protein processing machinery of the ER. Unlike Hsp110 and Hsp70, Grp170 has two distinct chaperoning domains; a classic beta-sheet peptide binding domain, with some COOH- terminal sequence, and a COOH-terminal alpha-helical domain. Like other members of the Hsp70 superfamily, Grp170 can elicit anti-tumor immune responses most likely by chaperoning antigenic peptide and binding to receptor(s) on antigen-presenting cells (APC).
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions ELISA: confirmed with antibody (1-10ug/ml) binding to Grp170 at 5ug/ml on the solid phase

Immunoblotting: with antibody at 1.0-2.0ug/ml. A band of 170kDa is detected.

These are recommended concentrations

End user should determine optimal concentrations for their applications.

Positive control: HEK293 cell lysate.
References & Data Sheet