Anti-ENaC β Antibody (11577)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,IHC Mouse
SKU: 11577 Categories: , ,
Overview
Product Name Anti-ENaC β Antibody (11577)
Description Anti-ENaC β Mouse Monoclonal Antibody
Target ENaC β
Species Reactivity Mouse
Applications WB,IHC
Host Mouse
Clonality Monoclonal
Clone ID 1.60E+05
Isotype IgG2a
Immunogen Synthetic peptide corresponding to C-terminal aa 617-638 of rat ENaC alpha.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity Mouse ENaC beta
Target Name Amiloride-sensitive sodium channel subunit β
Target ID ENaC β
Uniprot ID P37090
Alternative Names β-NaCH, Epithelial Na(+ channel subunit β, β-ENaC, Nonvoltage-gated sodium channel 1 subunit β, SCNEB
Gene Name Scnn1b
Accession Number NP_036780
Sequence Location Apical cell membrane, Cytoplasmic vesicle membrane
Biological Function Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride (PubMed:9118951). Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception. {UniProtKB:P51168, PubMed:9118951}.
Research Areas Ion Channels
Background The epithelial sodium channel (ENaC) is a membrane-bound ion-channel that is selectively permeable to Na+ ions and that is assembled as a heterotrimer composed of three homologous subunits alpha, beta, and g. It is involved primarily in the reabsorption of sodium ions in the collecting ducts of the kidney's nephrons. These channels mediate the first step of active sodium reabsorption essential for the maintenance of body salt and water homeostasis. In vertebrates, the channels control reabsorption of sodium in kidney, colon, lung and sweat glands; they also play a role in taste perception.
Application Images
Description Immunohistochemistry analysis using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8 (11577). Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody (11577) at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.
Description Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8 (11577). Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 µg. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody (11577) at 1:1000. Predicted/Observed Size: ~87kDa.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet