Anti-Choline acetyltransferase Antibody (56571)

$466.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ug WB,ICC/IF Human, Mouse
SKU: 56571 Categories: , ,
Overview
Product Name Anti-Choline acetyltransferase Antibody (56571)
Description Anti-Choline acetyltransferase Rabbit Polyclonal Antibody
Target Choline acetyltransferase
Species Reactivity Human, Mouse
Applications WB,ICC/IF
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to amino acids from the N-terminus to the mid-protein of human choline o-acetyltransferase.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by peptide immuno-affinity chromatography
Specificity Information
Specificity This antibody recognizes human and mouse choline acetyltransferase.
Target Name Choline O-acetyltransferase
Target ID Choline acetyltransferase
Uniprot ID P28329
Alternative Names CHOACTase, ChAT, Choline acetylase, EC 2.3.1.6
Gene Name CHAT
Biological Function Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. {PubMed:17144655}.
Research Areas Neuroscience
Background Choline acetyltransferase (ChAT) catalyzes the transfer of an acetyl group from acetyl-CoA to choline to produce acetylcholine. ChAT is found in high concentration in the central nervous system and peripheral nervous system. Alterations in concentrations of acetylcholine and ChAT have been associated with Alzheimer disease and Amyotrophic Lateral Sclerosis (ALS).
Application Images
Description Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (56571). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (56571) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Nucleus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) Choline Acetyltransferase Antibody (D) Composite.
Description Western blot analysis of Mouse Brain showing detection of ~82.5kDa Choline Acetyltransferase protein using Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (56571). Lane 1: MW Ladder. Lane 2: Mouse Brain (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Choline Acetyltransferase Polyclonal Antibody (56571) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~82.5kDa.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at dilution of 1:1,000. A band of ~80kDa is detected.

Immunofluorescence: use at dilution of 1:100.Detection of choline acetyltransferase in mouse brain lysate with #56571 diluted 1:1,000

These are recommended working dilutions.

Endusers should determine optimal dilutions for their applications.
References & Data Sheet