Anti-CENP-A Antibody (12597)

$346.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug WB,ELISA Human
SKU: 12597 Categories: , ,
Overview
Product Name Anti-CENP-A Antibody (12597)
Description Anti-CENP-A Mouse Monoclonal Antibody
Target CENP-A
Species Reactivity Human
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 5A7-2E11
Isotype IgG1
Immunogen Synthetic peptide corresponding to a sequence within human CENP-A.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by Protein G affinity chromatography
Specificity Information
Specificity This antibody recognizes human CENP-A. Reactivity with other species has not been investigated.
Target Name Histone H3-like centromeric protein A
Target ID CENP-A
Uniprot ID P49450
Alternative Names Centromere autoantigen A, Centromere protein A, CENP-A
Gene Name CENPA
Accession Number NP_001035891.1
Sequence Location Nucleus, Chromosome, centromere, kinetochore, Chromosome, centromere
Biological Function Histone H3-like nucleosomal protein that is specifically found in centromeric nucleosomes (PubMed:7962047, PubMed:9024683, PubMed:11756469, PubMed:14667408, PubMed:15702419, PubMed:15475964, PubMed:15282608, PubMed:17651496, PubMed:19114591, PubMed:27499292, PubMed:20739937). Replaces conventional H3 in the nucleosome core of centromeric chromatin at the inner plate of the kinetochore (PubMed:18072184). The presence of CENPA subtly modifies the nucleosome structure and the way DNA is wrapped around the nucleosome and gives rise to protruding DNA ends that are less well-ordered and rigid compared to nucleosomes containing histone H3 (PubMed:27499292, PubMed:26878239). May serve as an epigenetic mark that propagates centromere identity through replication and cell division (PubMed:15475964, PubMed:15282608, PubMed:26878239, PubMed:20739937, PubMed:21478274). Required for recruitment and assembly of kinetochore proteins, and as a consequence required for progress through mitosis, chromosome segregation and cytokinesis (PubMed:11756469, PubMed:14667408, PubMed:18072184, PubMed:23818633, PubMed:25556658, PubMed:27499292). {PubMed:11756469, PubMed:14667408, PubMed:15282608, PubMed:15475964, PubMed:15702419, PubMed:17651496, PubMed:18072184, PubMed:19114591, PubMed:21478274, PubMed:23818633, PubMed:25556658, PubMed:26878239, PubMed:27499292, PubMed:7962047, PubMed:9024683, PubMed:20739937}.
Research Areas DNA
Background Replicated chromosomes include two kinetochores that control chromosome segregation during mitosis. Centromere Protein-A (CENP-A), a histone H3-like protein, contains a C-terminal H3-like domain which is required for centromere localization of CENP-A. It is essential for kinetochore targeting of CENP-C. In the presence of DNA, CENP-A forms an octameric complex with histones H2A, H2B, and H4. CENP-A specifically localizes to active centromeres and is a component of specialized centromeric nucleosomes on which kinetochores are assembled. CENP-A is essential for nucleosomal packaging of centromeric DNA at interphase and functions as a centromere formation marker on chromosomes.
Application Images
Description Immunoblotting: use at 1ug/ml. A band of ~18kDa is detected. Detection of CENP-A in lysate of U2OS cells
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1ug/mL. A band of ~18kDa is detected.Detection of CENP-A in lysate of U2OS cellsELISA: use at 1-10ug/mL with CENP-A on the solid phase.

Positive control: U2OS cell lysate

These are recommended concentrations;

Enduser should determine optimal concentrations for their applications.
References & Data Sheet