Anti-CaMKII Antibody (56370-50)

$386.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 50ug WB Human, Mouse, Rat, Bovine, Hamster, Guinea Pig, Chicken, Rabbit
SKU: 56370-50 Categories: , ,
Overview
Product Name Anti-CaMKII Antibody (56370-50)
Description Anti-CaMKII Rabbit Polyclonal Antibody
Target CaMKII
Species Reactivity Human, Mouse, Rat, Bovine, Hamster, Guinea Pig, Chicken, Rabbit
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to aa 7-20 (N-terminus) of rat CaMKII delta isoform conjugated to KLH.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, 50% glycerol, 0.09% sodium azide.
Buffer Formulation Phosphate Buffered Saline
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by protein A affinity chromatography
Specificity Information
Target Name Calcium/calmodulin-dependent protein kinase type II subunitα
Target ID CaMKII
Alternative Names CaM kinase II subunitα, CaMK-II subunitα, EC 2.7.11.17
Gene Name CAMK2A
Gene ID 815
Accession Number NP_741960.1
Research Areas Enzymes
Background Calmodulin-dependent protein kinase II (CaMKII) is a serine/threonine-specific protein kinase that is regulated by the Ca2+/calmodulin complex. CaMKII is involved in many signaling cascades and is thought to be an important mediator of learning and memory. CaMKII is also necessary for Ca2+ homeostasis and reuptake in cardiomyocytes, chloride transport in epithelia, positive T-cell selection, and CD8 T-cell activation. CaMKII has 28 different isoforms that derive from the alpha, beta, gamma, and delta genes. Misregulation of CaMKII is linked to Alzheimer's disease, Angelman syndrome, and heart arrhythmia.
Application Images
Description Immunoblotting: Use at 1-5ug/ml. A band of ~50-60 kDa is detected. Detection of CaMKII in mouse brain extract (Lane 2), rat brain extract (Lane 3), HeLa cell lysate (Lane 4), Hs-67 cell lysate (Lane 5).
Description Immunohistochemistry: use at 1-10ug/ml. Detection of CaMKII in human lung.
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: Use at 1-5ug/mL. A band of ~50-60 kDa is detected.

Immunohistochemistry: use at 1-10ug/mL.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
References & Data Sheet