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Anti-Calreticulin Antibody (56259)

$313.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 100ul WB Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Monkey, Pig, Hamster, Rabbit, Sheep
SKU: 56259 Categories: , ,
Overview
Product Name Anti-Calreticulin Antibody (56259)
Description Anti-Calreticulin Antibody Rabbit Polyclonal Antibody
Target Calreticulin
Species Reactivity Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Monkey, Pig, Hamster, Rabbit, Sheep
Applications WB
Host Rabbit
Clonality Polyclonal
Immunogen Human calreticulin synthetic peptide conjugated to KLH.
Properties
Form Liquid
Concentration Lot Specific
Formulation Whole antiserum
Buffer Formulation Whole Antiserum
Format Whole Antiserum
Purification Whole antiserum
Specificity Information
Specificity This antibody recognizes human, mouse, rat, bovine, canine, chicken, guinea pig, monkey, pig, hamster, rabbit, and sheep calreticulin.
Target Name Calreticulin
Target ID Calreticulin
Uniprot ID P27797
Alternative Names CRP55, Calregulin, Endoplasmic reticulum resident protein 60, ERp60, HACBP, grp60
Gene Name CALR
Sequence Location Endoplasmic reticulum lumen, Cytoplasm, cytosol, Secreted, extracellular space, extracellular matrix, Cell surface, Sarcoplasmic reticulum lumen, Cytoplasmic vesicle, secretory vesicle, Cortical granule, Cytolytic granule
Biological Function Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity). {UniProtKB:P28491, UniProtKB:Q8K3H7, PubMed:11149926, PubMed:7876246}.
Research Areas Neuroscience
Background Calreticulin is a multifunctional highly conserved Ca+2-binding protein that is localized primarily in the endoplasmic reticulum (ER) but has also been detected in the nucleus and nuclear envelope. Like many other ER proteins, it has the conserved ER retention KDEL sequence (Lys-Asp-Glu-Leu) at its C-terminus. Calreticulin is made up of three domains: 1 180 residue N-terminal domain, a proline- rich P-domain (aa 189-288) that binds Ca+2 with high affinity and shares homology with calnexin and calmegin, and a 110 residue C-terminal domain that binds Ca+2 with low affinity but high capacity. Calreticulin appears to be involved in calcium storage and regulation, has molecular chaperone activity, and has been shown to be induced in response to various types of cell stress.
Application Images
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium which is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 1:5,000-1:10,000 dilution. A band of ~63 kDa is detected.

These are recommended concentrations. User should determine optimal concentrations for their application.

Positive control: HeLa cell lysate.
References & Data Sheet