Anti-Calcineurin A (Catalytic) Antibody (56336-200)

$591.00

Host Quantity Applications Species Reactivity Data Sheet  
Rabbit 200ug WB,IP Human, Mouse, Rat, Rabbit, Hamster, Bovine, Canine
SKU: 56336-200 Categories: , ,
Overview
Product Name Anti-Calcineurin A (Catalytic) Antibody (56336-200)
Description Anti-Calcineurin A (Catalytic) Rabbit Polyclonal Antibody
Target Calcineurin A (Catalytic)
Species Reactivity Human, Mouse, Rat, Rabbit, Hamster, Bovine, Canine
Applications WB,IP
Host Rabbit
Clonality Polyclonal
Immunogen Synthetic peptide corresponding to aa 264-283 of human Calcineurin A conjugated to KLH. This sequence is identical in rat and mouse calcineurin A.
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, 50% glycerol, 0.09% sodium azide
Buffer Formulation Phosphate Buffered Saline
Buffer Anti-Microbial 0.09% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Purified by protein A affinity chromatography
Specificity Information
Target Name Serine/threonine-protein phosphatase 2B catalytic subunitα isoform
Target ID Calcineurin A (Catalytic)
Alternative Names EC 3.1.3.16, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunitα isoform
Gene Name PPP3CA
Gene ID 5530
Accession Number NP_000935
Research Areas Neuroscience
Background Calcineurin is a Ca+2/calmodulin-dependent serine/threonine protein phosphatase. The active enzyme is a heterodimer consisting of a catalytic A subunit and a myristylated regulatory B subunit that binds calcium. It controls the translocation of nuclear factors from the cytosol into the nucleus of activated T cells. Calcineurin is a target of immunosuppressive drugs such as cyclosporin A and FK506.
Application Images
Description Detection of Calcineurin A in rat brain lysate (Lane 1) and mouse brain lysate (Lane 2).
Additional Information
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: Use at 1-5ug/mL. A band of ~61kDa is detected.

These are recommended concentrations.

Endusers should determine optimal concentrations for their application.
References & Data Sheet