Anti-BRD2 Antibody (13020)

$518.00

Host Quantity Applications Species Reactivity Data Sheet  
Goat 100ug WB,IP Human
SKU: 13020 Categories: , ,
Overview
Product Name Anti-BRD2 Antibody (13020)
Description Anti-BRD2 Goat Polyclonal Antibody
Target BRD2
Species Reactivity Human
Applications WB,IP
Host Goat
Clonality Polyclonal
Isotype Whole IgG
Immunogen Synthetic peptide representing a portion of the protein between the putative nuclear localization signal and the ET domain
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
Buffer Formulation Tris
Buffer pH pH 7.8
Buffer Anti-Microbial 0.1% Sodium Azide
Format Purified
Purification Purified by immunoaffinity chromatography
Specificity Information
Specificity This antibody reacts with human BRD2.The specific epitope is in the region between aa 550 and 600 of human BRD2.Based on 100% sequence identity, this antibody is predicted to react with Bovine, Rhesus Monkey, Gorilla, White-tufted-ear marmoset, Crab-eating macaque and Northern white-cheeked gibbon.Antibody is affinity-purified with an epitope specific to BRD2 immobilized on solid support.
Target Name Bromodomain-containing protein 2
Target ID BRD2
Uniprot ID P25440
Alternative Names O27.1.1, Really interesting new gene 3 protein
Gene Name BRD2
Accession Number NP_001106653
Sequence Location Nucleus
Biological Function May play a role in spermatogenesis or folliculogenesis (By similarity). Binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling. Regulates transcription of the CCND1 gene. Plays a role in nucleosome assembly. {PubMed:18406326}.
Research Areas Enzymes
Background BRD2 (bromodomain-containing 2) is a nuclear mitogen-activated kinase that plays a role in transcription of cell-cycle- regulated genes by functioning as an adapter protein that recruits E2F and chromatin remodeling factors to E2F response elements.
Application Images
Description Detection of human BRD2 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified goat anti-BRD2 antibody 13020 (lot 13020-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Additional Information
Handling
Storage This product is stable at 2 -8°C for 1 year.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: 0.025 - 0.1 ug/mL.Detection of human BRD2 in RIPA extract (10ug) of HeLa cells untreated (-) or treated (+) with calf intestinal phosphatase with #13020 at 0.05ug/mL.
References & Data Sheet