Anti-β lactamase Antibody (15720)

$230.00 – $460.00

Host Quantity Applications Species Reactivity Data Sheet  
Mouse 100ug
1mg
500ug 		WB,ELISA E. Coli
SKU: 15720 Categories: , ,
Overview
Product Name Anti-β lactamase Antibody (15720)
Description Anti-β lactamase Mouse Monoclonal Antibody
Target β-Lactamase
Species Reactivity E. Coli
Applications WB,ELISA
Host Mouse
Clonality Monoclonal
Clone ID 8A5.A10
Isotype IgG1
Immunogen 5'-His-tagged E. coli 205 TEM-1 R+ beta-lactamase.
Immunogen Sequence MSIQHFRVAL IPFFAAFCLP VFAHPETLVK VKDAEDQLGA RVGYIELDLN SGKILESFRP EERFPMMSTF KVLLCGAVLS RVDAGQEQLG RRIHYSQNDL VEYSPVTEKH LTDGMTVREL CSAAITMSDN TAANLLLTTI GGPKELTAFL HNMGDHVTRL DRWEPELNEA IPNDERDTTM PAAMATTLRK LLTGELLTLA SRQQLIDWME ADKVAGPLLR SALPAGWFIA DKSGAGERGS RGIIAALGPD GKPSRIVVIY TTGSQATMDE RNRQIAEIGA SLIKHW
Properties
Form Liquid
Concentration Lot Specific
Formulation PBS, pH 7.4
Buffer Formulation Phosphate Buffered Saline
Buffer pH 7.4
Format Purified
Purification Purified by protein G affinity chromatography
Specificity Information
Target Name β-lactamase
Target ID β-Lactamase
Uniprot ID Q6SJ61
Gene Name TEM-1
Gene ID 10076131
Accession Number NP_943295.1
Research Areas Enzymes
Application Images
Description Nontypeable Haemophilus influenzae (NTHi) isolated from the patient’s throat culture secretes Beta-lactamase-carrying OMVs, which are induced by amoxicillin (NTHi OMVsBetaL+AMX+): (a) electron microscopy of negatively stained NTHi OMVsBetaL+AMX+; scale bar, 200 nm. (b) OMV immunoblot with anti-Beta-lactamase antibody. Left panel: lane 1, NTHi OMVsBetaL+AMX+, lane 2, control Beta-lactamase-negative OMVs (NTHi OMVsBetaL−), lane 3, protein size marker (the marker band sizes are on the right side); Beta-lactamase is ~32 kDa. Right panel: OMVsBetaL+AMX+ subjected to proteinase K (PK) assay, which demonstrates intravesicular localization of Beta-lactamase. (c,d) Beta-lactamase activities (c) and the amounts (d) of OMVs produced by NTHi freshly isolated from the patient’s throat culture (fresh isolate), by NTHi passaged twice in BHI broth without amoxicillin (P1 and P2, AMX-), and by NTHi from passage 2 grown in BHI broth with amoxicillin concentrations reported in the tonsils during therapy (0.17 µg/mL, 1.1 µg/mL, or 3.9 µg/mL). Data are presented as means ± standard deviations from three independent experiments; ** p < 0.01 compared to fresh isolate; xx p < 0.01 compared to P2 AMX-; xxx p < 0.001 compared to P2 AMX- (statistical analysis was performed with one-way ANOVA).
Additional Information
Handling
Storage These antibodies are stable for at least one (1) year at -20°C to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions Immunoblotting: use at 10ug/ml. Predicted molecular weight ~29kDa

ELISA: use at 10-20ug/ml (optimized for immobilized beta-lactamase at 10ug/ml).

End users should determine optimal concentrations for their applications.
References & Data Sheet
References In Vivo Secretion of β-Lactamase-Carrying Outer Membrane Vesicles as a Mechanism of β-Lactam Therapy Failure. Membranes (Basel) (2021) [34832035]