Anti-Bcl-2 (Phospho-Ser70) Antibody (43065)
$366.00
| Host | Quantity | Applications | Species Reactivity | Data Sheet | |
|---|---|---|---|---|---|
| Rabbit | 100ul | IHC | Human |  |
Overview
Product Name Anti-Bcl-2 (Phospho-Ser70) Antibody (43065)
Description Anti-Bcl-2 (Phospho-Ser70) Rabbit Polyclonal Antibody
Target Bcl-2 (Phospho-Ser70)
Species Reactivity Human
Applications IHC
Host Rabbit
Clonality Polyclonal
Immunogen Peptide sequence that includes phosphorylation site of Serine 70 (R-T-S(p)-P-L) derived from human Bcl-2 and conjugated to KLH.
Properties
Form Liquid
Concentration 1.0 mg/mL
Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Buffer Formulation Phosphate Buffered Saline
Buffer pH pH 7.4
Buffer Anti-Microbial 0.02% Sodium Azide
Buffer Cryopreservative 50% Glycerol
Format Purified
Purification Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Specificity Information
Specificity This antibody detects endogenous human Bcl-2 only when phosphorylated at serine 70.Accession no.: P10415, NP_000624.2
Target Name Apoptosis regulator Bcl-2
Target ID Bcl-2 (Phospho-Ser70)
Uniprot ID P10415
Gene Name BCL2
Gene ID 151430
Post Translational Modification Bcl-2 (Phospho-Ser70)
Target Phospho-ser70
Sequence Location Mitochondrion outer membrane, Nucleus membrane, Endoplasmic reticulum membrane, Cytoplasm
Biological Function Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells (PubMed:1508712, PubMed:8183370). Regulates cell death by controlling the mitochondrial membrane permeability (PubMed:11368354). Appears to function in a feedback loop system with caspases (PubMed:11368354). Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1) (PubMed:11368354). Also acts as an inhibitor of autophagy: interacts with BECN1 and AMBRA1 during non-starvation conditions and inhibits their autophagy function (PubMed:18570871, PubMed:21358617, PubMed:20889974). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). {PubMed:1508712, PubMed:17418785, PubMed:18570871, PubMed:20889974, PubMed:21358617, PubMed:8183370, PubMed:11368354}.
Research Areas Phosphospecific Antibodies
Background Bcl-2 protein suppresses apoptosis in a variety of cellular systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspase. Bcl-2 inhibits caspase acativity either by preventing release of cytochrome c from the mitochondria and/or by binding to apoptosis-activating factor (Apaf-1). The predicted molecular weight of Bcl-2 is ~26kDa.
Handling
Storage This antibody is stable for at least one (1) year at -20°C.
Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.
Application Instructions
Immunohistochemistry: use at dilution of 1:50-1:100.
These are recommended working dilutions.
End user should determine optimal dilutions for their application.
Immunohistochemistry: use at dilution of 1:50-1:100.
These are recommended working dilutions.
End user should determine optimal dilutions for their application.
References & Data Sheet
Data Sheet 
Download PDF Data Sheet
Download PDF Data Sheet