Anti-ALIEN Antibody (41010)

$376.00

Host	Quantity	Applications	Species Reactivity	Data Sheet
Rabbit	100ug	WB	Human, Drosophila

SKU: 41010 Categories: Antibody Products, Growth Factors/Cytokines/Receptor Antibodies, Products

Overview

Product Name Anti-ALIEN Antibody (41010)

Description Anti-Alien Rabbit Polyclonal Antibody

Target ALIEN

Species Reactivity Human, Drosophila

Applications WB

Host Rabbit

Clonality Polyclonal

Immunogen Human Alien protein.

Properties

Form Liquid

Concentration Lot Specific

Formulation PBS, pH 7.4.

Buffer Formulation Phosphate Buffered Saline

Buffer pH pH 7.4

Format Purified

Purification Purified by immunoaffinity chromatography

Specificity This antibody recognizes human and Drosophila Alien, a co-repressor protein that interacts with the thyroid hormone receptor in the absence of hormone. Alien is highly conserved between human and Drosophila and is specific for selected members of the nuclear hormone receptor superfamily.

Target Name COP9 signalosome complex subunit 2

Target ID ALIEN

Uniprot ID P61201

Alternative Names SGN2, Signalosome subunit 2, Alien homolog, JAB1-containing signalosome subunit 2, Thyroid receptor-interacting protein 15, TR-interacting protein 15, TRIP-15

Gene Name COPS2

Sequence Location Cytoplasm, Nucleus

Biological Function Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Involved in early stage of neuronal differentiation via its interaction with NIF3L1. {PubMed:11285227, PubMed:11337588, PubMed:12628923, PubMed:12732143, PubMed:9535219}.

Research Areas Growth Factors, Cytokines, Receptors

Handling

Storage This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Dilution Instructions Dilute in PBS or medium that is identical to that used in the assay system.

Application Instructions Immunoblotting: use at 1:500-1:1,000 dilution. In immunoblots, a band of approximately 50 kD is detected.

Data Sheet